Relationship between intra- and extracellular pH in primary cultures of rat astrocytes.
Relationship between intra- and extracellular pH in primary cultures of rat astrocytes.
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大鼠星形胶质细胞原代培养物中细胞内和细胞外 pH 值的关系。
DOI：
10.1152/ajpcell.1994.267.2.c581
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Siesjo,BK
中科院分区：
文献类型：
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作者：
Mellergard,P;Ou-Yang,Y;Siesjo,BK
采用单细胞显微荧光技术和pH敏感荧光团2 '，7'-bis（2-carboxyethyl）-5（6）-carboxyfluorescein研究了细胞外pH（pHe）对培养的新生大鼠皮层星形胶质细胞调节细胞内pH（pHi）的影响。当在由增加的CO2张力引起的酸瞬变期间将pHe维持在7.35的控制值时，pHi被迅速调节回到正常。然而，在pHe 6.9或更低时，pHi没有恢复。稳态pHi也强烈依赖于pHe（pHi = 1.14 + 0.80 pHe）。pHi恢复正常后的pHe是非常迅速的，表明长期暴露于低pH刺激pH调节机制，并抑制4，4 '-二异硫氰基芪-2，2'-二磺酸（DIDS）和阿米洛利或Na+的去除。Cd ~（2+）的加入也使恢复速度减慢。DIDS或阿米洛利对pH依赖性酸化没有严重影响，河豚毒素、四乙基铵、Ba 2+、2，3-二羟基-6-硝基-7-氨磺酰基-苯并（F）-喹喔啉、犬尿喹啉酸、5-羟基苯氯酚、荷包牡丹碱、Cd 2+或白蛋白也不抑制pH依赖性酸化。结果强调的重要性，pH值的调节，并表明，在星形胶质细胞中的pH值不调节回正常水平，直到pH值正常化。
We studied the influence of extracellular pH (pHe) on the mechanisms regulating intracellular pH (pHi) in astrocytes cultured from neonatal rat cortex, using single cell microspectrofluorometry and the pH-sensitive fluorophore 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein. When pHe was maintained at control values of 7.35 during acid transients caused by an increased CO2 tension, pHi was rapidly regulated back to normal. However, at pHe 6.9 or below, there was no recovery of pHi. Steady-state pHi was also strongly dependent on pHe (pHi = 1.14 + 0.80 pHe). The pHi recovery after normalization of pHe was very rapid, indicating that a prolonged exposure to a low pH stimulates pH-regulating mechanisms, and was inhibited by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) and amiloride or the removal of Na+. Recovery was also slowed down by Cd2+. The pHe-dependent acidification was not critically influenced by DIDS or amiloride and was not inhibited by tetrodotoxin, tetraethylammonium, Ba2+,2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(F)-quinoxaline, kynurenic acid, 5-hydroxysaclofen, bicuculline, Cd2+, or albumin. The results emphasize the importance of pHe for pHi regulation and suggest that in astrocytes pHi is not regulated back to normal levels until pHe is normalized.
水蛭中枢神经系统中已鉴定的神经胶质细胞和神经元对细胞内 pH 值的调节。
DOI：
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发表时间：
1987
期刊：
Journal of Physiology
影响因子：
--
作者：
J. W. Deitmer;W. Schlue
通讯作者：
W. Schlue
DOI：
10.1073/pnas.80.1.165
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
DEAMER, DW;NICHOLS, JW
通讯作者：
NICHOLS, JW