Chemical synthesis of lymphotactin: a glycosylated chemokine with a C-terminal mucin-like domain.
Chemical synthesis of lymphotactin: a glycosylated chemokine with a C-terminal mucin-like domain.
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DOI：
10.1002/1521-3765(20010302)7:5
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发表时间：
2001-03
期刊：
影响因子：
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通讯作者：
L. Marcaurelle;L. Mizoue;J. Wilken;Lisa Oldham;S. Kent;T. Handel;C. Bertozzi
中科院分区：
文献类型：
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作者：
L. Marcaurelle;L. Mizoue;J. Wilken;Lisa Oldham;S. Kent;T. Handel;C. Bertozzi
利用天然化学结扎技术合成了含有8个o -链糖基化位点的93个残基趋化因子淋巴趋化素。使用标准的fmoc化学将单个GalNAc残基结合到每个糖基化位点，以实现粘蛋白型糖蛋白的首次全合成。使用这种方法获得了大量的均质材料进行结构和功能分析。
The synthesis of a 93-residue chemokine, lymphotactin, containing eight sites of O-linked glycosylation, was achieved using the technique of native chemical ligation. A single GalNAc residue was incorporated at each glycosylation site using standard Fmoc-chemistry to achieve the first total synthesis of a mucin-type glycoprotein. Using this approach quantities of homogeneous material were obtained for structural and functional analysis.