A CARBOHYDRATE SIDE-CHAIN ON HEMAGGLUTININS OF HONG-KONG INFLUENZA-VIRUSES INHIBITS RECOGNITION BY A MONOCLONAL-ANTIBODY
A CARBOHYDRATE SIDE-CHAIN ON HEMAGGLUTININS OF HONG-KONG INFLUENZA-VIRUSES INHIBITS RECOGNITION BY A MONOCLONAL-ANTIBODY
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DOI：
10.1073/pnas.81.6.1779
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
WILEY, DC
中科院分区：
文献类型：
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作者：
SKEHEL, JJ;STEVENS, DJ;WILEY, DC
在1968年香港（H3）流感病毒的抗原变体的血凝素中检测到一个单一的氨基酸取代，即Asp-63至Asn-63，该变体是通过在小鼠单克隆抗体存在下生长野生型病毒而选择的。该突变在残基63-65处产生糖基化的寡糖附着位点Asn-Cys-Thr。免疫沉淀实验与提取物从变异病毒感染的鸡胚成纤维细胞制备的衣霉素，抑制糖基化的存在或不存在下，证明了新的寡糖侧链的添加是必要的，以防止与单克隆抗体的反应。用1969年香港流感流行的病毒A/England/878/69进行的类似实验也支持这一结论，并为碳水化合物介导的血凝素抗原性修饰的流行病学意义提供了证据。
A single amino acid substitution, Asp-63 to Asn-63, was detected in the hemagglutinin of an antigenic variant of the 1968 Hong Kong (H3) influenza virus that was selected by growth of the wild-type virus in the presence of a mouse monoclonal antibody. The mutation generates an oligosaccharide attachment site, Asn-Cys-Thr at residues 63-65, that is glycosylated. Immunoprecipitation experiments with extracts from variant virus-infected chicken embryo fibroblast cells prepared in the presence or absence of tunicamycin, which inhibits glycosylation, demonstrate that addition of the new oligosaccharide side chain is required to prevent reaction with the monoclonal antibody. Similar experiments with the virus of the 1969 Hong Kong influenza epidemic, A/England/878/69, which also contains a hemagglutinin glycosylated at residue 63, support this conclusion and provide evidence for the epidemiological significance of carbohydrate-mediated modifications of hemagglutinin antigenicity.