Methods for isolation of marine-derived endophytic fungi and their bioactive secondary products
Methods for isolation of marine-derived endophytic fungi and their bioactive secondary products
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DOI：
10.1038/nprot.2009.233
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发表时间：
2010-01-01
期刊：
影响因子：
14.8
通讯作者：
Proksch, Peter
中科院分区：
文献类型：
--
作者：
Kjer, Julia;Debbab, Abdessamad;Proksch, Peter
近年来，海洋来源的真菌已显示出产生大量新的生物活性次生代谢物，其中一些具有迄今为止自然界中前所未有的新碳框架。这些化合物作为新的先导结构在医药和植物保护方面具有重要意义。本协议的目的是详细描述分离和培养与各种海洋生物（海绵、藻类和红树林植物）相关的真菌的方法，用于提取、表征和阐明这些海洋内生真菌产生的生物活性次级代谢物的结构，以及基于快速“内部”筛选系统对其药理学特性进行初步评估。最后给出了一些结果，说明了该方案的积极效果。从海洋环境取样到完成结构解析和生物活性筛选，至少需要3个月的时间。
Marine-derived fungi have been shown in recent years to produce a plethora of new bioactive secondary metabolites, some of them featuring new carbon frameworks hitherto unprecedented in nature. These compounds are of interest as new lead structures for medicine as well as for plant protection. The aim of this protocol is to give a detailed description of methods useful for the isolation and cultivation of fungi associated with various marine organisms (sponges, algae and mangrove plants) for the extraction, characterization and structure elucidation of biologically active secondary metabolites produced by these marine-derived endophytic fungi, and for the preliminary evaluation of their pharmacological properties based on rapid 'in house' screening systems. Some results exemplifying the positive outcomes of the protocol are given at the end. From sampling in marine environment to completion of the structure elucidation and bioactivity screening, a period of at least 3 months has to be scheduled.