Protein Production Using the Baculovirus-Insect Cell Expression System
Protein Production Using the Baculovirus-Insect Cell Expression System
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DOI：
10.1002/btpr.1842
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发表时间：
2014-01-01
影响因子：
2.9
通讯作者：
Chisti, Y.
中科院分区：
文献类型：
--
作者：
Contreras-Gomez, A.;Sanchez-Miron, A.;Chisti, Y.
杆状病毒-昆虫细胞表达系统广泛用于生产重组蛋白。本综述的重点是该表达系统在开发生产感兴趣蛋白质的生物过程中的用途。解决的问题包括：杆状病毒生物学和遗传操作以提高蛋白质表达和质量；抑制与病毒酶相关的蛋白水解作用；对昆虫细胞系进行工程改造，以提高表达蛋白质的糖基化和折叠能力；杆状病毒对宿主细胞的影响及其对蛋白质生产的影响；生长培养基对宿主细胞代谢的影响；生物反应器和相关操作方面；以及产品的下游加工。所有这些因素都强烈影响重组蛋白的生产。回顾了当前的知识状况。 (c) 2013 年美国化学工程师生物技术研究所。编, 30:1-18, 2014
The baculovirus-insect cell expression system is widely used in producing recombinant proteins. This review is focused on the use of this expression system in developing bioprocesses for producing proteins of interest. The issues addressed include: the baculovirus biology and genetic manipulation to improve protein expression and quality; the suppression of proteolysis associated with the viral enzymes; the engineering of the insect cell lines for improved capability in glycosylation and folding of the expressed proteins; the impact of baculovirus on the host cell and its implications for protein production; the effects of the growth medium on metabolism of the host cell; the bioreactors and the associated operational aspects; and downstream processing of the product. All these factors strongly affect the production of recombinant proteins. The current state of knowledge is reviewed. (c) 2013 American Institute of Chemical Engineers Biotechnol. Prog., 30:1-18, 2014