LIPIDS OF INCOMPLETE INFLUENZA VIRUS
LIPIDS OF INCOMPLETE INFLUENZA VIRUS
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DOI：
10.1016/0042-6822(70)90213-8
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发表时间：
1970-01-01
期刊：
影响因子：
3.7
通讯作者：
MERLIE, JP
中科院分区：
文献类型：
--
作者：
BLOUGH, HA;MERLIE, JP
对标准流感病毒（A0/PR8/34）和冯·马格努斯型不完整病毒进行了分析和比较。在连续未稀释传代条件下繁殖的病毒具有比标准病毒更低的胆固醇-磷脂摩尔比(0.6)。磷脂的比较显示，鞘磷脂和磷脂酰胆碱的量减少，而磷脂酰乙醇胺的量增加。极性脂质部分的脂肪酸谱揭示了向短链酸和长链多不饱和脂肪酸的转变。通过一维双溶剂系统分离的中性脂质表明，不完整病毒的甘油酯含量增加，并向甘油单酯和甘油二酯转变。这种变化往往会改变流感病毒脂质的包装，并导致包膜更加膨胀和流动——这解释了冯·马格努斯型病毒包膜的多形性和缺陷。
Standard influenza virus (A0/PR8/34) and incomplete virus of the von Magnus type were analyzed and compared. Virus propagated under conditions of serial undiluted passage had a lower molar ratio of cholesterol-phospholipid (0.6) than did standard virus. A comparison of phospholipids revealed decreased amounts of sphingomyelin and phosphatidylcholine together with increased amounts of phosphatidylethanolamine. Fatty acid profiles of the polar lipid fractions revealed a shift toward short-chain acids and long-chain polyunsaturated fatty acids. Neutral lipids, separated by a unidimensional, two-solvent system, revealed that incomplete virus had an increased amount of glycerides with a shift toward mono- and diglycerides. Such changes would tend to alter the packing of the lipids of influenza virus and lead to a more expanded and fluid envelope—accounting for the pleomorphism and defects in the envelope of the von Magnus type virus.