Dynamics of DNA methylation and Histone H4 acetylation during floral bud differentiation in azalea.
Dynamics of DNA methylation and Histone H4 acetylation during floral bud differentiation in azalea.
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DOI：
10.1186/1471-2229-10-10
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发表时间：
2010-01-12
影响因子：
5.3
通讯作者：
Cañal MJ
中科院分区：
文献类型：
--
作者：
Meijón M;Feito I;Valledor L;Rodríguez R;Cañal MJ
在杜鹃花等观赏植物中，控制开花时间的能力是规划生产的关键策略，但它需要对花的过渡有透彻的了解。花的转变是通过复杂的遗传网络实现的，并受到多种环境和内源因素的调节。染色质状态之间的动态变化促进或抑制DNA转录，以响应环境和发育信号调节花诱导途径的表达。DNA甲基化和组蛋白修饰参与控制染色质的功能状态和基因表达。研究结果表明，杜鹃花顶芽从营养发育到生殖发育的过程中，DNA甲基化和组蛋白H4乙酰化等表观遗传机制具有相反的、特殊的动态。DNA甲基化和组蛋白H4乙酰化的全球水平，以及5-mdC和乙酰化H4的免疫检测，以及形态学研究，使杜鹃花芽发育的四个基本阶段得以划分，并使表观遗传重编程的一个阶段得以确定，该阶段显示整个DNA甲基化的急剧下降，类似于植物和哺乳动物的其他发育过程。表观遗传控制和染色质重组似乎对杜鹃花的协调发育起着决定性的作用。在烟叶顶端分生组织发育和花分化过程中，DNA甲基化和H4去乙酰化同时协调作用，重构染色质，调控基因表达。
The ability to control the timing of flowering is a key strategy for planning production in ornamental species such as azalea, however it requires a thorough understanding of floral transition. Floral transition is achieved through a complex genetic network and regulated by multiple environmental and endogenous cues. Dynamic changes between chromatin states facilitating or inhibiting DNA transcription regulate the expression of floral induction pathways in response to environmental and developmental signals. DNA methylation and histone modifications are involved in controlling the functional state of chromatin and gene expression. The results of this work indicate that epigenetic mechanisms such as DNA methylation and histone H4 acetylation have opposite and particular dynamics during the transition from vegetative to reproductive development in the apical shoots of azalea. Global levels of DNA methylation and histone H4 acetylation as well as immunodetection of 5-mdC and acetylated H4, in addition to a morphological study have permitted the delimitation of four basic phases in the development of the azalea bud and allowed the identification of a stage of epigenetic reprogramming which showed a sharp decrease of whole DNA methylation similar to that is defined in other developmental processes in plants and in mammals. The epigenetic control and reorganization of chromatin seem to be decisive for coordinating floral development in azalea. DNA methylation and H4 deacetylation act simultaneously and co-ordinately, restructuring the chromatin and regulating the gene expression during soot apical meristem development and floral differentiation.