Resonance Raman studies on pyrocatechase
Resonance Raman studies on pyrocatechase
复制标题
焦儿茶酶的共振拉曼研究
DOI：
10.1021/ja00502a057
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发表时间：
1979
影响因子：
15
通讯作者：
R. Heistand
中科院分区：
文献类型：
--
作者：
L. Que;R. Heistand
0.062±0.002 s-1。在几分钟的时间跨度内没有看到光谱的进一步变化。K+跳跃实验也显示420-nm峰向340-nm吸收的缓慢转化，但动力学不是干净的一级动力学。从短时间内的数据可以得到0.3-0.5 s-1的近似一级速率常数，但由于吸光度的连续变化，分析变得复杂。方案I，或修改版本，包括Na+-K+交换的影响，占主要观察到的光谱变化。然而，其他的变化也会发生，这将需要添加到这个简单的现象学方案。对这些数据的全面分析，包括使用主成分技术14正在进行中，这将进一步加深我们对聚糖酶催化机制的理解。
of 0.062±0.002 s-1. No further changes in the spectrum were seen over a time span of several minutes. The K+-jump experiment also showed slow conversion of the 420-nm peak to the 340-nm absorption, but the kinetics are not cleanly first order. An approximate first-order rate constant of 0.3-0.5 s-1 can be obtained from the data at short times, but the analysis is complicated by continuing changes in absorb-ance. Scheme I, or a modified versionto include the effects of Na+-K+ exchange, accounts for the major observed spectral changes. However, other changes also occur which will require additions to this simple phenomenological scheme. Full anal-ysis of these data including the use of principal component techniques14 is underway and should further our understanding of the mechanism of tryptophanase catalysis.