Evaluation of bacterial association in methane generation pathways of an anaerobic digesting sludge via metagenomic sequencing
Evaluation of bacterial association in methane generation pathways of an anaerobic digesting sludge via metagenomic sequencing
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DOI：
10.1007/s00203-019-01716-x
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发表时间：
2020-01-01
影响因子：
2.8
通讯作者：
Wang, Kaijun
中科院分区：
文献类型：
--
作者：
Ali, Nasir;Gong, Hui;Wang, Kaijun
厌氧消化是最近热门的一种生产沼气的技术，特别是从废水中产生甲烷以生产生物燃料，被认为是解决能源危机和全球污染威胁的有效解释。污泥中存在复杂的微生物群，其在复杂聚合物消化成简单单体的过程中发挥着重要作用。由于涉及极端复杂的群体，16S rRNA 方法根本不足以进行扩增。然而，Illumina 测序是一项最新的强大技术，可以揭示厌氧消化中的整个微生物组结构和甲烷生成途径。通过宏基因组测序揭示了北京当地污水处理厂消化污泥的微生物结构。 Illumina HiSeq 程序用于提取约 5 GB 的数据用于宏基因组分析。 MG-RAST 服务器分类调查显示，细菌优势率约为 97.64%，古细菌占 1.78%。据报道，最丰富的细菌群落是放线菌门、拟杆菌门、厚壁菌门和变形菌门。此外，还揭示了参与甲烷生成的重要微生物组。检测到了显性产甲烷菌（Methanosaeta 和 Methanosarcina），与消化污泥中参与乙酰分解产甲烷作用的显性基因有关联。宏基因组分析表明，厌氧消化器中的微生物结构和甲烷生成途径已成功剖析。
Anaerobic digestion, a recently hot technology to produce biogases especially methane generation for biofuel from wastewater, is considered an effective explanation for energy crisis and global pollution threat. A complex microbiome population is present in sludge, which plays an important role in the digestion of complex polymer into simple monomers. 16S rRNA approaches simply are not enough for amplification due to the involvement of extreme complex population. However, Illumina sequencing is a recent powerful technology to reveal the entire microbiome structure and methane generation pathways in anaerobic digestion. Metagenomic sequencing was tested to reveal the microbial structure of a digested sludge from a local wastewater treatment plant in Beijing. The Illumina HiSeq program was used to extract about 5 GB of data for metagenomic analysis. The classification investigation revealed about 97.64% dominancy of bacteria while 1.78% were detected to be archaea using MG-RAST server. The most abundant bacterial communities were reported to be Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria. Furthermore, the important microbiome involved in methane generation was revealed. The dominant methanogens were detected (Methanosaeta and Methanosarcina), with affiliation of dominant genes involved in acetoclastic methanogenesis in a digesting sludge. The metagenomic analysis showed that microbial structure and methane generation pathways were successfully dissected in an anaerobic digester.