Phenolic acid contents of kale (Brassica oleraceae L. var. acephala DC.) extracts and their antioxidant and antibacterial activities
Phenolic acid contents of kale (Brassica oleraceae L. var. acephala DC.) extracts and their antioxidant and antibacterial activities
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DOI：
10.1016/j.foodchem.2007.07.003
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发表时间：
2008-03-01
期刊：
影响因子：
8.8
通讯作者：
Strnad, Miroslav
中科院分区：
文献类型：
--
作者：
Ayaz, Faik Ahmet;Hayirlioglu-Ayaz, Sema;Strnad, Miroslav
通过HPLC-MS鉴定了羽衣甘蓝（黑甘蓝）叶片中的9种酚酸和种子中的10种酚酸。游离酚酸、酯酚酸（甲醇可溶）、糖苷酚酸和酯结合酚酸（甲醇不溶）含量分别为487、532、4989和6402 ng/g鲜重。阿魏酸和咖啡酸（总含量; 4269和4887纳克/克，分别）是最丰富的。这些馏分的种子含量分别为1993，1477，1231和4909纳克/克干重（DW），芥子酸是最丰富的（5037纳克/克DW）。馏分的总酚含量，测定比色法，高度相关的DPPH清除能力，并在抗微生物活性测定，与9个测试生物代表了广泛的分类群，所有的馏分是有效的金黄色葡萄球菌，粪肠球菌，枯草芽孢杆菌和（最强烈）卡他莫拉氏菌。讨论了羽衣甘蓝酚类化合物在游离和共轭形式下的抗菌和抗氧化活性。(C)2007爱思唯尔有限公司保留所有权利。
Nine phenolic acids were identified and quantified by HPLC-MS in leaves and 10 in seeds of kale (black cabbage). The free, ester (methanol-soluble), glycoside and ester-bound (methanol-insoluble) phenolic acid contents of the leaves were 487, 532, 4989 and 6402 ng/g fresh weight, respectively. Ferulic and caffeic acids (total contents; 4269 and 4887 ng/g, respectively) were the most abundant. The seed contents of these fractions were 1993, 1477, 1231 and 4909 ng/g dry weight (DW), respectively, and sinapic acid was the most abundant (5037 ng/g DW). The fractions' total phenolic contents, determined colorimetrically, were highly correlated with their DPPH scavenging capacity, and in antimicrobial activity assays, with nine test organisms representing a wide array of taxa, all of the fractions were effective against Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis and (most strongly) Moraxella catarrhalis. Antimicrobial and antioxidant activities of kale phenolics in free and conjugated forms are discussed. (C) 2007 Elsevier Ltd. All rights reserved.