Circular RNA expression alterations in extracellular vesicles isolated from murine heart post ischemia/reperfusion injury
Circular RNA expression alterations in extracellular vesicles isolated from murine heart post ischemia/reperfusion injury
复制标题
缺血/再灌注损伤后从小鼠心脏分离的细胞外囊泡中环状RNA表达的变化
DOI：
10.1016/j.ijcard.2019.08.024
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发表时间：
2019-12-01
影响因子：
3.5
通讯作者：
Zhou, Xiaohui
中科院分区：
文献类型：
--
作者：
Ge, Xinyu;Meng, Qingshu;Zhou, Xiaohui
Background: Increasing studies indicated the involvement of extracellular vesicles (EVs) in cardiovascular diseases. However, the role of circular RNAs (circRNAs) in cardiac EVs (cEVs) during ischemia/reperfusion (I/R) injury remain unclear.Methods: We isolated the cEVs from I/R injured hearts and performed RNA sequencing (RNA-seq) to identify the profile of circRNA in cEVs and investigated their potential roles in I/R pathological process.Results: Cardiac I/R induced a significantly elevated release of EVs in heart within 24 h. RNA-seq of cEVs identified 185 significantly differentially expressed (DE) circRNAs including 119 down-regulated and 66 up-regulated circRNAs in I/R group compared with the sham. GO and pathway analysis showed that these DE-circRNAs were associated with protein binding and kinase activator activity and mainly involved in the metabolic process. The circRNA-miRNA analysis exhibited the broad potentials of the DE-circRNAs to regulate target genes by acting on the miRNAs.Conclusions: These findings revealed for the first time the specific expression pattern of circRNAs in EVs derived from sham and I/R heart tissues and provided some potential targets and pathways involving in I/R injury which may provide important evidences for the role of both circRNA and EVs in the pathology of cardiac I/R. (C) 2019 Elsevier B.V. All rights reserved.
