Albumin modulates the production of matrix metalloproteinases-2 and-9 in podocytes
Albumin modulates the production of matrix metalloproteinases-2 and-9 in podocytes
复制标题
白蛋白调节足细胞中基质金属蛋白酶-2和-9的产生
DOI：
10.1007/s11596-009-0607-1
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发表时间：
2009-12-01
影响因子：
--
通讯作者：
Zhang, Chun
中科院分区：
文献类型：
--
作者：
Fang, Zhan;He, Fangfang;Zhang, Chun
To investigate the effects of albumin on the production of matrix metalloproteinases-2 (MMP-2) and matrix metalloproteinases-9 (MMP-9) in podocytes. Podocytes were treated with bovine serum albumin (BSA) at the concentration of 0.1, 0.5, 1, 2 g/L, respectively. Conditioned media were harvested 12, 24, 48 and 72 h after the treatment. The expression of MMP-2 and MMP-9 was assayed by gelatin zymography, RT-PCR and Western blotting analysis. Our results showed that in comparison with the control group, BSA increased the expression of MMP-2 and MMP-9 mRNA and protein in a dose- and time-dependent manner (P<0.05). Meanwhile, the enzymatic activities of MMP-2 and MMP-9 in the culture supernatants of podocytes were also increased (P<0.05). It is concluded that albumin up-regulated the expression of MMP-2 and MMP-9 at gene and protein levels in a time- and dose-dependent manner.
