gga-microRNA-375 negatively regulates the cell cycle and proliferation by targeting Yes-associated protein 1 in DF-1 cells
gga-microRNA-375 negatively regulates the cell cycle and proliferation by targeting Yes-associated protein 1 in DF-1 cells
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gga-microRNA-375 通过靶向 DF-1 细胞中的 Yes 相关蛋白 1 负向调节细胞周期和增殖
DOI：
10.3892/etm.2020.8711
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发表时间：
2020
影响因子：
2.7
通讯作者：
Xie Qingmei
中科院分区：
文献类型：
--
作者：
Zhang Xinheng;Liao Zhihong;Wu Yu;Yan Yiming;Chen Sheng;Lin Shaoli;Chen Feng;Xie Qingmei
microRNAs（miRNAs/miRs）在调节细胞周期和诱导肿瘤发生中起关键作用。J亚群禽白血病病毒（ALV-J）属于逆转录病毒科、正逆转录病毒亚科和α逆转录病毒属，其在易感鸡中引起肿瘤。在ALV-J诱导的鸡肝脏肿瘤中，gga-miR-375下调，YAP 1上调，并且已经进一步确定YAP 1是gga-miR-375的直接靶基因。本研究发现ALV-J感染能促进DF-1细胞的增殖和细胞周期的变化。由于细胞周期和细胞增殖与肿瘤发生密切相关，因此进行了进一步的实验以确定gga-miR-375和YAP 1是否参与这些细胞过程。结果表明，gga-miR-375通过抑制G1期向S/G2期的转化，抑制细胞增殖，从而显著抑制细胞周期，而YAP 1则显著促进细胞周期和增殖。此外，gga-miR-375通过靶向YAP 1影响DF-1细胞中的这些细胞过程。总的来说，本研究结果表明，ALV-J感染下调的gga-miR-375通过靶向YAP 1负调控细胞周期和增殖。
MicroRNAs (miRNAs/miRs) serve a key role in regulating the cell cycle and inducing tumorigenesis. Subgroup J of the avian leukosis virus (ALV-J) belongs to the family Retroviridae, subfamily Orthoretrovirinae and genus Alpharetrovirus that causes tumors in susceptible chickens. gga-miR-375 is downregulated and Yes-associated protein 1 (YAP1) is upregulated in ALV-J-induced tumors in the livers of chickens, and it has been further identified that YAP1 is the direct target gene of gga-miR-375. In the present study, it was found that ALV-J infection promoted the cell cycle and proliferation in DF-1 cells. As the cell cycle and cell proliferation are closely associated with tumorigenesis, further experiments were performed to determine whether gga-miR-375 and YAP1 were involved in these cellular processes. It was demonstrated that gga-miR-375 significantly inhibited the cell cycle by inhibiting G1 to S/G2 stage transition and decreasing cell proliferation, while YAP1 significantly promoted the cell cycle and proliferation. Furthermore, these cellular processes in DF-1 cells were affected by gga-miR-375 through the targeting of YAP1. Collectively, the present results suggested that gga-miR-375, downregulated by ALV-J infection, negatively regulated the cell cycle and proliferation via the targeting of YAP1.