Production of Cyanobacterial Hepatotoxin Microcystin in a Nutrient-limited Culture of Microcystis viridis.
Production of Cyanobacterial Hepatotoxin Microcystin in a Nutrient-limited Culture of Microcystis viridis.
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绿色微囊藻营养限制培养物中蓝藻肝毒素微囊藻毒素的生产。
DOI：
10.2521/jswtb.38.39
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Y. Inamori
中科院分区：
文献类型：
--
作者：
Naoshi Fujimoto;M. Soma;Maiko Yoshida;Masaharu Suzuki;R. Takahashi;Y. Inamori
研究了绿色微囊藻（Microcystis viridis）在营养盐梯度培养条件下产生微囊藻毒素的情况。微囊藻毒素的比产生速率随硝酸盐和磷酸盐浓度的增加而增加。硝酸盐供应后，硝酸盐饥饿的培养物的比生产率高于磷酸盐供应后，磷饥饿的培养物，显示氮是一个更重要的营养微囊藻毒素生产比磷。以2 h为间隔，研究了硝酸盐胁迫下绿色微囊藻产生微囊藻毒素的时间进程。在24小时内，微囊藻毒素-RR含量增加到2倍以上。在24小时内没有观察到干重的增加，表明微囊藻毒素在氮饥饿的M的细胞生长之前产生。绿色的微囊藻毒素RR的比生成速率随水体中硝酸盐细胞定额的增加而增加，说明水体中的硝酸盐细胞定额可能对微囊藻毒素的生成速率有一定的调节作用。
The production of microcystin by a nutrient-starved Microcystis viridis culture with a nutrient gradient was examined. The specific production rate of microcystin increased with increasing nitrate and phosphate concentrations. The specific production rates of nitrate-starved cultures after nitrate supply were higher than those of phosphate-starved cultures after phosphate supply showing that nitrogen is a more important nutrient for microcystin production than phosphorus. The time course of microcystin production by nitrate-starved M.viridis after nitrate supply was evaluated at 2h intervals. The microcystin-RR content increased to more than 2-fold within 24 hrs. An increase in dry weight was not observed within 24 hrs, showing microcystin was produced prior to cell growth for nitrogen-starved M. viridis. The specific production rate of microcystin-RR seemed to increase with increasing nitrate cell quota, which suggested that the nitrogen cell quota might regulate the rate of production of microcystin in lakes and reservoirs.