Effect of biosurfactant on cellulase and xylanase production by Trichoderma viride in solid substrate fermentation
Effect of biosurfactant on cellulase and xylanase production by Trichoderma viride in solid substrate fermentation
复制标题
生物表面活性剂对绿色木霉固体基质发酵中纤维素酶和木聚糖酶生产的影响
DOI：
10.1016/j.procbio.2006.05.014
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发表时间：
2006-11-01
影响因子：
4.4
通讯作者：
Chen, Shi
中科院分区：
文献类型：
--
作者：
Liu, Jia;Yuan, Xingzhong;Chen, Shi
研究了生物表面活性剂鼠李糖脂对绿色木霉固体发酵生产纤维素酶和木聚糖酶的影响。并与化学表面活性剂吐温80进行了比较。结果表明，不同浓度的鼠李糖脂对Avicelase、CMCase和纤维二糖酶的活性均有不同程度的增强作用。在产酶高峰期，鼠李糖脂和吐温80使纤维素酶活性提高20- 50%。而在发酵后期，添加鼠李糖脂后，Avicelase和Cellobiase的酶活分别比对照提高了122.6%和157.6%。鼠李糖脂和吐温80对产酶高峰期的木聚糖酶有明显的促进作用。添加0.018%（w/w）的鼠李糖脂酶活最高，比对照提高了119.6%。总的来说，鼠李糖脂的刺激作用上级优于吐温80。(c)2006爱思唯尔有限公司版权所有。
The effects of biosurfactant rhamnolipid were tested on the production of cellulase and xylanase from Trichoderma viride in solid substrate fermentation (SSF). The effects of rhamnolipid were also compared with those of chemical surfactant Tween 80. The results showed that rhamnolipid at different concentrations enhanced activities of Avicelase, CMCase and cellobiase to different extents. During the peak enzyme production phase, rhamnolipid and Tween 80 increased cellulase activities by 20-50%. However, in the later fermentation phase, the activities of Avicelase and cellobiase in the presence of rhamnolipid were 122.6% and 157.6% higher than those of the control, respectively. Both rhamnolipid and Tween 80 had the pronounced stimulatory effects on xylanase during the peak enzyme production. Rhamnolipid at 0.018% (w/w) gave the highest xylanase activity, which was 119.6% higher than the control. In the whole, the stimulatory effects of rhamnolipid were superior to those of Tween 80. (c) 2006 Elsevier Ltd. All rights reserved.