Chikungunya virus of Asian and Central/East African genotypes in Malaysia
Chikungunya virus of Asian and Central/East African genotypes in Malaysia
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DOI：
10.1016/j.jcv.2009.07.016
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发表时间：
2009-10-01
影响因子：
8.8
通讯作者：
AbuBakar, Sazaly
中科院分区：
文献类型：
--
作者：
Sam, I-Ching;Chan, Yoke Fun;AbuBakar, Sazaly
背景：中非/东非基因型基孔肯雅病毒（CHIKV）近年来在世界范围内引起了大规模暴发。在马来西亚，1998年和2006年报告了亚洲地方性和输入性中非/东非基因型的有限CHIKV暴发。自2008年4月以来，一场前所未有的全国性疫情影响了马来西亚。目的：研究当前马来西亚CHIKV暴发的分子流行病学，并评估感染患者血清对亚洲和中/东非基因型分离株的交叉中和活性。研究设计：从2008年就诊的83例患者中收集血清样本，用PCR检测El基因、病毒分离和IgM。对全长837 bp的部分El基因序列进行了系统发育分析。对本次暴发和蒲甘潘乔尔暴发（2006年亚洲基因型）的恢复期血清进行了交叉中和活性检测，以检测其对每次暴发的代表性菌株的交叉中和活性。结果：确诊34例（41.0%）。当前暴发的菌株在El结构蛋白中具有A226V突变，并与最近全球暴发的中非/东非分离株归为一类。血清中/东非和亚洲基因型交叉中和活性在40至1280滴度范围内观察。结论：引起马来西亚最大疫情的CHIKV毒株为中非/东非基因型。A226V突变增强了白纹伊蚊传播CHIKV病毒的可能性，这可能是寨卡病毒在农村地区广泛传播的原因。不同基因型的血清交叉中和可能有助于潜在的疫苗，并限制未来疫情的影响。(C) 2009 Elsevier B.V.版权所有
Background: Chikungunya virus (CHIKV) of the Central/East African genotype has caused large outbreaks worldwide in recent years. In Malaysia, limited CHIKV outbreaks of the endemic Asian and imported Central/East African genotypes were reported in 1998 and 2006. Since April 2008, an unprecedented nationwide outbreak has affected Malaysia.Objective: To study the molecular epidemiology of the current Malaysian CHIKV outbreak, and to evaluate cross-neutralisation activity of serum from infected patients against isolates of Asian and Central/East African genotypes.Study design: Serum samples were collected from 83 patients presenting in 2008, and tested with PCR for the El gene, virus isolation, and for IgM. Phylogenetic analysis was performed on partial El gene sequences of 837 bp length. Convalescent serum from the current outbreak and Bagan Panchor outbreak (Asian genotype, 2006) were tested for cross-neutralising activity against representative strains from each outbreak.Results: CHIKV was confirmed in 34 patients (41.0%). The current outbreak strain has the A226V mutation in the El structural protein, and grouped with Central/East African isolates from recent global outbreaks. Serum cross-neutralisation activity against both Central/East African and Asian genotypes was observed at titres from 40 to 1280.Conclusions: The CHIKV strain causing the largest Malaysian outbreak is of the Central/East African genotype. The presence of the A226V mutation, which enhances transmissibility of CHIKV by Aedes albopictus, may explain the extensive spread especially in rural areas. Serum cross-neutralisation of different genotypes may aid potential vaccines and limit the effect of future outbreaks. (C) 2009 Elsevier B.V. All rights reserved.