Differential proteomic analysis of white adipose tissues from T2D KKAy mice by LC-ESI-QTOF
Differential proteomic analysis of white adipose tissues from T2D KKAy mice by LC-ESI-QTOF
复制标题
通过 LC-ESI-QTOF 对 T2D KKAy 小鼠白色脂肪组织进行差异蛋白质组分析
DOI：
10.1002/pmic.201600219
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发表时间：
2017-03-01
期刊：
影响因子：
3.4
通讯作者：
Deng, Yunlin
中科院分区：
文献类型：
--
作者：
Ke, Ming;Wu, Hanyan;Deng, Yunlin
2型糖尿病(T2D)因其高发病率和高患病率，已成为世界范围内日益严重的社会健康负担。Wat作为胰岛素刺激下摄取葡萄糖的主要组织之一，在代谢和稳态调节中起着非常重要的作用。采用O-18标记的相对定量技术，对高脂饲料喂养的T2D KKAy小鼠和正常喂养的C57BL/6J小鼠的附睾脂肪垫进行了差异蛋白质组学研究。在确定的329个蛋白质中，121个蛋白质以Cv2或-lt；0.5为阈值发生显著变化。根据GO分类，我们发现改变的蛋白质包含代谢过程、氧化应激、离子动态平衡、细胞凋亡和细胞分裂等生物过程的成员。代谢中，分配给脂肪酸生物合成的蛋白质(Fas等)。β-氧化过程中的关键酶(ACOX3)升高。增加糖酵解酶(EnOB等)降低TCA循环相关酶(SCOT1等)。提示T2D小鼠线粒体糖代谢可能受损。氧化应激升高，表现为一系列氧化脱氢酶(QSOX1等)的改变。此外，离子动态平衡的改变(AT2c1等)此外，还观察到蛋白质的存在。促进细胞增殖相关蛋白(Elys等)和抑制细胞凋亡相关蛋白(RASF6等)。Wat可能会导致脂肪垫和体重增加。综上所述，WAT的这些变化可能为理解T2D的作用机制提供参考。
Type 2 diabetes (T2D) has become a worldwide increasingly social health burden for its high morbidity and heightened prevalence. As one of the main tissues involved in uptake of glucose under the stimulation of insulin, WAT plays very important role in metabolic and homeostasis regulation. We performed a differential proteomics study to investigate alterations in epididymis fat pad of high fat diet fed T2D KKAy mice compared to normal fed C57BL/6J mice, by O-18-labeling relative quantitative technique. Among 329 confidently identified proteins, 121 proteins showed significant changes with CV 2 or < 0.5 as threshold). According to GO classification, we found that altered proteins contained members of biological processes of metabolic process, oxidative stress, ion homeostasis, apoptosis and cell division. In metabolic, proteins assigned to fatty acid biosynthesis (FAS etc.) were decreased, the key enzyme (ACOX3) in beta-oxidation process was increased. Increased glycolysis enzymes (ENOB etc.) and decreased TCA cycle related enzymes (SCOT1 etc.) suggested that glucose metabolism in mitochondria of T2D mice might be impaired. Elevated oxidative stress was observed with alterations of a series of oxidordeuctase (QSOX1 etc.). Besides, alterations of ion homeostasis (AT2C1 etc.) proteins were also observed. The enhancement of cell proliferation associated proteins (ELYS etc.) and inhibition of apoptosis associated proteins (RASF6 etc.) in WAT might contributed to the fat pad and body weight gain. Overall, these changes in WAT may serve as a reference for understanding the functional mechanism of T2D.