Rapid detection and high occurrence of porcine rotavirus A, B, and C by RT-qPCR in diagnostic samples
Rapid detection and high occurrence of porcine rotavirus A, B, and C by RT-qPCR in diagnostic samples
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DOI：
10.1016/j.jviromet.2014.08.018
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发表时间：
2014-12-01
影响因子：
3.1
通讯作者：
Ciarlet, Max
中科院分区：
文献类型：
--
作者：
Marthaler, Douglas;Homwong, Nitipong;Ciarlet, Max
轮状病毒是包括人类在内的动物腹泻的重要原因。目前，已在猪身上发现轮状病毒A、B、C、E和H(RVA-RVC、RVE和RVH)。传统上，RVA被认为是引起猪腹泻的主要原因，直到最近，RVB和RVC在猪中才被零星描述。设计了定性的猪RVA、RVB和RVC RT-PCR(RT-qPCR)方法，并对提交给明尼苏达大学的7508份猪腹泻样本进行了测试，以估计在大约两年的时间(从2009年到2011年)内RVA、RVB和RVC的百分比。单独的RVA和RVC RT-qPCR检测被多重为单一的RT-qPCR，而RVB RT-qPCR检测仍然是单独的RT-qPCR。总体而言，83%的样本RVA、RVB或RVC呈阳性。正如预期的那样，RVA的总体检测百分比最高(62%)。然而，分别有33%和53%的样本RVB和RVC呈阳性，这表明RVB和RVC在猪群中也具有重要的流行病学意义。RVC在1~20日龄仔猪中占优势，而RVA和RVB在21日龄猪中占优势。作为诊断工具，开发的RT-qPCR分析可以成功地区分感染的轮状病毒种类，这可能导致更好的监测和流行病学研究，最终制定更好的预防和控制策略。(C)2014爱思唯尔B.V.保留所有权利。
Rotaviruses are important cause of diarrhea in animals, including humans. Currently, rotavirus species A, B, C, E, and H (RVA-RVC, RVE, and RVH) have been identified in pigs. Traditionally, RVA has been considered the primary cause of diarrhea in pigs, and RVB and RVC had been described sporadically in pigs until recently. Qualitative porcine RVA, RVB, and RVC RT-PCR (RT-qPCR) assays were designed and 7508 porcine diarrheic samples, submitted to University of Minnesota, were tested to estimate the percentage of RVA, RVB, and RVC over a period of approximately 2 years (from 2009 to 2011). The individual RVA and RVC RT-qPCR assays were multiplex into a single RT-qPCR while the RVB RT-qPCR assay remained as an individual RT-qPCR. In total, 83% of the samples were positive for RVA, RVB, or RVC. As expected, RVA was detected at the highest overall percentage (62%). However, 33% and 53% of the samples were positive for RVB and RVC, respectively, indicating that both RVB and RVC are also epidemiologically important in the swine population. RVC was most predominant in young pigs (1-20 days of age), while RVA and RVB were most predominant in >= 21 day old pigs. As diagnostic tools, the developed RT-qPCR assays could successfully discriminate among infecting RV species, which could lead to better surveillance and epidemiological studies for ultimately better prevention and control strategies. (C) 2014 Elsevier B.V. All rights reserved.