Evidence of neurotrophic events due to peritoneal endometriotic lesions
Evidence of neurotrophic events due to peritoneal endometriotic lesions
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DOI：
10.1016/j.cyto.2013.03.003
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发表时间：
2013-05-01
期刊：
影响因子：
3.8
通讯作者：
Mechsner, Sylvia
中科院分区：
文献类型：
--
作者：
de Arellano, Maria Luisa Barcena;Arnold, Julia;Mechsner, Sylvia
为了研究子宫内膜异位症的神经营养特性，以及神经营养因子在子宫内膜异位症患者慢性盆腔疼痛发展中的作用，我们进行了一项前瞻性临床研究。研究了腹膜炎性病变或子宫腺肌病患者、非子宫炎性粘连妇女和无子宫内膜异位症/子宫腺肌病/粘连妇女的腹膜液（PF）中神经营养因子的存在。腹膜炎性病变患者的PF分为三组：无症状子宫内膜异位症，轻微疼痛和严重疼痛。将子宫腺肌病患者、非子宫内膜粘连患者和对照组的PF分为无痛和无痛两组。用ELISA和培养的鸡感觉神经节（背根神经节，DRG）和交感神经节的神经元生长试验分析PF中神经营养因子的表达。腹膜增生性病变妇女的PF过度表达神经生长因子（NGF）和神经营养因子-3（NT-3），但不表达脑源性神经营养因子（BDNF），而子宫腺肌病或粘连妇女的PF似乎表达正常量的这些因子。神经营养因子的表达在疼痛组之间没有差异。此外，PF腹膜炎病变的患者诱导了强烈的感觉和边缘交感神经突起的生长，而PF子宫腺肌病和非腹膜炎粘连的妇女诱导了类似于对照组的生长。仅在与腹膜增生病变孵育的DRG中，诱导的轴突生长才能被抑制。有趣的是，交感神经节的生长在所有研究groups.The本研究表明，只有腹膜炎病变导致增加释放的NGF和NT-3到PP和NGF调节神经纤维的生长在子宫内膜异位症。(C)2013爱思唯尔有限公司保留所有权利。
To investigate the neurotrophic properties of endometriosis, as well as the involvement of neurotrophic factors in the development of chronic pelvic pain in patients with endometriosis, we performed a prospective clinical study. The presence of neurotrophins was investigated in the peritoneal fluid (PF) of patients with peritoneal endometriotic lesions or adenomyosis, as well as from women with non-endometriotic adhesions and from women without endometriosis/adenomyosis/adhesions. The PF from patients with peritoneal endometriotic lesions was divided in three groups: asymptomatic endometriosis, minimal pain and severe pain. PF from patients with adenomyosis or with non-endometriotic adhesions and the control group were divided in patients without pain and with pain. Neurotrophin expression in PF was analyzed using Elisa and the neuronal growth assay with cultured chicken sensory ganglia (dorsal-root-ganglia, DRG) and sympathetic ganglia. PF from women with peritoneal endometriotic lesions overexpress nerve growth factor (NGF) and neurotrophin-3 (NT-3), but not brain derived neurotrophic factor (BDNF), whereas the PF of women with adenomyosis or adhesions seems to express normal amounts of these factors. Neurotrophin expression did not differ among the pain groups. Furthermore, the PF from patients with peritoneal endometriotic lesions induced a strong sensory and a marginal sympathetic neurite outgrowth, while the PF from women with adenomyosis and non-endometriotic adhesions induced an outgrowth similar to the control group. The induced neurite outgrowth could only be inhibited in DRG incubated with peritoneal endometriotic lesions. Interestingly, the outgrowth of sympathetic ganglia was inhibited in all studied groups.The present study suggests that only peritoneal endometriotic lesions lead to an increased release of NGF and NT-3 into the PP and that NGF modulates the nerve fiber growth in endometriosis. (C) 2013 Elsevier Ltd. All rights reserved.