Variation of immunoglobulins G, A, and M and bovine serum albumin concentration in Holstein cow colostrum
Variation of immunoglobulins G, A, and M and bovine serum albumin concentration in Holstein cow colostrum
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DOI：
10.1016/j.animal.2021.100299
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发表时间：
2021-06-22
期刊：
影响因子：
3.6
通讯作者：
De Marchi, M.
中科院分区：
文献类型：
--
作者：
Costa, A.;Goi, A.;De Marchi, M.
免疫球蛋白G（IgG）、A（伊加）和M（IgM）占牛初乳中总蛋白的70-80%，是抗体从母体被动转移到小牛所必需的。考虑到初乳样品采集的实际困难和分析的高成本，三种免疫球蛋白组分的非遗传变异来源几乎没有一起在奶牛中进行大规模研究。在本研究中，IgG，伊加，IgM，和牛血清白蛋白（BSA）的荷斯坦奶牛初乳样品中测定通过牛特异性放射免疫扩散试剂盒，这样的表型允许调查的奇偶校验，牛群，产犊季节，和相互作用的影响。本研究仅考虑第一次初乳，因为小牛出生后立即与母体分离，不允许哺乳。IgG（n = 676）、伊加（n = 573）、IgM（n = 658）、总免疫球蛋白（n = 525）和BSA（n = 614）的平均值分别为91.31、4.20、105.99、5.05和2.47 g/L，各性状之间均呈正相关。总的来说，免疫球蛋白在第一次和第二次产次奶牛的初乳中的浓度低于后期产次奶牛。这些发现表明，基于IG的初乳质量在经历两次以上泌乳的奶牛中总体上更高，这可能是由于与年轻奶牛相比，免疫系统的经验更丰富，免疫遗传更广泛。至于产犊季节的影响，浓度的所有IG往往是一般较高的初乳采样从8月至11月。此外，IgG，伊加和IgM浓度之间存在差异的9个畜群。未来的研究将调查这些性状与产量的关系，以及牛初乳的总体和详细组成，并将考虑其遗传背景，以评估潜在的选择策略，以提高初乳质量。(c)2021作者（S）由Elsevier B. V.代表动物联盟出版。这是一个在CC BY-NC-ND许可证下的开放获取文章（http：//creativecommons.org/licenses/by-nc-nd/4.0/）。
Immunoglobulins G (IgG), A (IgA), and M (IgM) represent 70-80% of total proteins in cattle colostrum and are essential for the passive transfer of antibodies from the dam to the calf. Considering the practical difficulties of colostrum sample collection and the high cost of analysis, non-genetic sources of variation of the three immunoglobulins fractions have been scarcely studied together on a large scale in dairy cows. In the present study, IgG, IgA, IgM, and bovine serum albumin (BSA) were determined in colostrum samples of Holstein cows through bovine-specific radial immunodiffusion kits; such phenotypes allowed to investigate the effects of parity, herd, and calving season, and interactions. Only the first colostrum was considered in the present study, as the calf was separated from the dam immediately after birth and was not allowed to suckle. The average of IgG (n = 676), IgA (n = 573), IgM (n = 658), total immunoglobulins (n = 525), and BSA (n = 614) was 91.31, 4.20, 105.99, 5.05, and 2.47 g/L, respectively, and all traits positively correlated to each other. Overall, the immunoglobulins were less concentrated in colostrum of first- and second-parity cows than later-parity cows. These findings suggest that colostrum quality, based on Ig, is overall greater in cows that experienced more than two lactations, likely due to a greater experience of the immune system and to a wider immune heritage compared to younger cows. As regards the effect of calving season, the concentration of all Ig tended to be generally greater in colostrum sampled from August to November. Moreover, there were differences in IgG, IgA, and IgM concentration among the nine herds involved. Future studies will investigate the relationships of these traits with yield, and gross and detailed composition of bovine colostrum and will consider their genetic background to evaluate potential selection strategies to improve colostrum quality. (c) 2021 The Author(s). Published by Elsevier B.V. on behalf of The Animal Consortium. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).