Protective effect of trans-resveratrol against kainic acid-induced seizures and oxidative stress in rats
Protective effect of trans-resveratrol against kainic acid-induced seizures and oxidative stress in rats
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DOI：
10.1016/s0091-3057(01)00663-3
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发表时间：
2002-01-01
影响因子：
3.6
通讯作者：
Chaudhary, G
中科院分区：
文献类型：
--
作者：
Gupta, YK;Briyal, S;Chaudhary, G
兴奋性氨基酸的过度兴奋是癫痫发生的重要机制，其中自由基最近被认为起着关键作用。因此，抗氧化剂的干预可能是治疗癫痫的一种潜在的有益方法。本研究旨在观察高效抗氧化剂白藜芦醇对红藻氨酸诱导的癫痫发作的影响，以及对脑内氧化应激标志物的影响。海人酸，10毫克/公斤ip，诱导持久的癫痫发作和相关症状。发现与对照组（195 +/-26 nmol/g湿组织）相比，在红藻氨酸给药后，脑丙二醛（MDA）水平显著升高（295 +/-18 nmol/g湿组织）。预处理（5 min）单剂量的traps-resveratrol（40 mg/kg ip）不能抑制惊厥，但潜伏期明显延长。当在不同动物中以两个剂量方案（20和40 mg/kg ip，5分钟前和红藻氨酸后30和90分钟重复）注射多个剂量的白藜芦醇时，在两个治疗方案中惊厥的发生率显著降低。发现与单独的红藻氨酸相比，在陷阱-白藜芦醇处理组（20和40 mg/kg的多次剂量）中脑MDA水平显著减弱。然而，谷胱甘肽水平在控制，红藻氨酸和陷阱白藜芦醇治疗的动物没有显着差异。陷阱-白藜芦醇对红藻氨酸诱导的惊厥和MDA水平的衰减的保护作用表明抗氧化剂至少作为辅助治疗癫痫的潜在用途。(C)2002年爱思唯尔科学公司All rights reserved.
Overexcitation of excitatory amino acid is an important mechanism in seizure genesis wherein free radicals have recently been suggested to play a critical role. Thus, intervention by antioxidants can be a potential beneficial approach in the treatment of epilepsy. The present study was undertaken to see the effect of traps-resveratrol, a potent antioxidant, against kainic acid-induced seizures, and effect on markers of oxidative stress in brain. Kainic acid, 10 mg/kg ip, induced long-lasting seizures and associated symptoms. The brain level of malondialdehyde (MDA) was found to be significantly raised after kainic acid administration (295 +/- 18 nmol/g wet tissue) as compared to control (195 +/- 26 nmol/g wet tissue). Pretreatment (5 min) of single dose of traps-resveratrol (40 mg/kg ip) could not inhibit the convulsions though the latency was significantly increased. When multiple doses of tracts-resveratrol were injected in two-dose schedules in different animals (20 and 40 mg/kg ip, 5 min prior and repeated 30 and 90 min after kainic acid), there was significant reduction in incidence of convulsions in both treatment schedules. The brain MDA levels were found to be significantly attenuated in the traps-resveratrol-treated groups (multiple doses of 20 and 40 mg/kg) as compared to the kainic acid alone. However, the glutathione level in control, kainic acid- and traps-resveratrol-treated animals were not significantly different. The protective effect of traps-resveratrol against kainic acid-induced convulsions and the attenuation of raised MDA level suggest the potential use of antioxidants at least as adjunct therapy in epilepsy. (C) 2002 Elsevier Science Inc. All rights reserved.