Rotavirus and Hepatitis A virus in market lettuce (Latuca sativa) in Costa Rica
Rotavirus and Hepatitis A virus in market lettuce (Latuca sativa) in Costa Rica
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DOI：
10.1016/s0168-1605(97)00058-5
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发表时间：
1997-07-22
影响因子：
5.4
通讯作者：
Taylor, L
中科院分区：
文献类型：
--
作者：
Hernandez, F;Monge, R;Taylor, L
我们评估了在哥斯达黎加的圣何塞农贸市场购买的生菜中轮状病毒和甲型肝炎病毒的存在，分别在轮状病毒相关腹泻的低发病率（4月至6月）和高发病率（12月至1月）。合并样本，通过酶联免疫吸附试验（ELISA）和放射免疫分析，聚合酶链反应（PCR）和电子显微镜的甲型肝炎病毒轮状病毒进行评估。三个样本池，在腹泻的高患病率期间收集的，阳性轮状病毒ELISA和其中一个轮状病毒可视化的电子显微镜。在同一时期收集的两个样本池通过PCR显示甲型肝炎病毒呈阳性。在几乎所有池中，通过培养检测到粪大肠菌群，并通过电子显微镜观察到噬菌体。(C)1997年Elsevier Science B.V.
We assessed the presence of rotavirus and Hepatitis A virus in lettuce bought in farmer markets from San Jose, Costa Rica, during months of low (April-June) and high (December-January) incidence of diarrhea associated with rotavirus, respectively. Lettuce samples were pooled and evaluated for rotavirus by enzyme-linked immunosorbent assay (ELISA) and for Hepatitis A virus by radioimmunoassay, polymerase chain reaction (PCR) and electron microscope. Three sample pools, collected during the period of high prevalence of diarrhea, were positive for rotavirus by ELISA and in one of them rotavirions were visualized by electron microscopy. Two samples pools collected during the same period were positive for Hepatitis A virus as shown by PCR. In almost all the pools fecal coliform bacteria were detected by cultivation and bacteriophages were visualized by electron microscopy. (C) 1997 Elsevier Science B.V.