CE of phytosiderophores and related metal species in plants
CE of phytosiderophores and related metal species in plants
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DOI：
10.1002/elps.200700117
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发表时间：
2007-10-01
期刊：
影响因子：
2.9
通讯作者：
Weber, Gunther
中科院分区：
文献类型：
--
作者：
Xuan, Yue;Scheuermann, Enrico B.;Weber, Gunther
植物铁载体（PS）和密切相关的物质烟酰胺（NA）是禾本科植物吸收金属的关键物质。这里展示了这些物质和相关金属物质的 CE 分离。特别是，使用 pH 7.3 的 MES/Tris 缓冲液分离三种 PS 2-脱氧麦根酸 (DMA)、麦根酸 (MA) 和 3-表羟基麦根酸 (epi-HMA) 以及 NA。此外，不同 PS 的三种 Fe(III) 物种得以分离，没有任何稳定性问题，而这些问题在色谱分析中经常出现。还用相同的方法分离具有配体 DMA 和 NA 的 Cu、Ni 和 Zn 的二价金属物质。通过使用特殊的两性离子 CE 毛细管，甚至可以分离两种异构体 Fe(III) 螯合物与配体乙二胺-N,N'-双（邻羟基苯基）乙酸 (EDDHA)（即内消旋 Fe(III)-EDDHA 和外消旋 Fe(III)-EDDHA），并且为了快速分离 NA 以及相应的二价和三价金属物质，可以使用具有抑制 EOF 的聚合物 CE 微芯片被描述。所提出的CE方法适用于真实植物样品，并且能够检测与生物过程直接相关的金属种类（Cu-DMA、Ni-NA）的变化。
Phytosiderophores (PS) and the closely related substance nicotianamine (NA) are key substances in metal uptake into graminaceous plants. Here, the CE separation of these substances and related metal species is demonstrated. In particular, the three PS 2-deoxymugineic acid (DMA), mugineic acid (MA), and 3-epi-hydroxymugineic acid (epi-HMA), and NA, are separated using MES/Tris buffer at pH 7.3. Moreover, three Fe(III) species of the different PS are separated without any stability problems, which are often present in chromatographic analyses. Also divalent metal species of Cu, Ni, and Zn with the ligands DMA and NA are separated with the same method. By using a special, zwitterionic CE capillary, even the separation of two isomeric Fe(III) chelates with theligandethylenediamine-N,N'-bis(o-hydroxyphenyl)acetic acid (EDDHA) is possible (i.e., meso-Fe(III)-EDDHA and rac-Fe(III)-EDDHA), and for fast separations of NA and respective divalent and trivalent metal species, a polymer CE microchip with suppressed EOF is described. The proposed CE method is applicable to real plant samples, and enables to detect changes of metal species (Cu-DMA, Ni-NA), which are directly correlated to biological processes.