INFLUENCE OF INORGANIC PHOSPHATE IN THE FORMATION OF PHOSPHATASES BY ESCHERICHIA-COLI
INFLUENCE OF INORGANIC PHOSPHATE IN THE FORMATION OF PHOSPHATASES BY ESCHERICHIA-COLI
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DOI：
10.1016/0006-3002(60)91281-6
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发表时间：
1960-01-01
期刊：
影响因子：
--
通讯作者：
TORRIANI, A
中科院分区：
文献类型：
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作者：
TORRIANI, A
酸性和碱性磷酸酶已在大肠杆菌菌株中得到证实。杆菌细菌酸性磷酸酶（最适pH 4-5）对磷酸己糖显示出相当高的特异性，而碱性酶（最适pH 8.5-9.5）水解所有测试的磷酸单酯。已经研究了两种酶形成的动力学，并且已经表明，仅当Pi在培养基中变得受限时才形成可测量量的碱性磷酸酶，此时酶以最大速率大量形成。控制酶dormation的机制方面的研究结果的影响进行了讨论。
Both acid and alkaline phosphatase have been demonstrated in strains of E. coli. Bacterial acid phosphatase (pH optimum 4-5) displays a fairly high specificity for the hexosephosphates, while the alkaline enzyme (pH optimum 8.5-9.5) hydrolyzes all the phosphomonoesters tested. The kinetics of formation of both enzymes have been studied and it has been shown that alkaline phosphatase in measurable amount is only formed when Pi becomes limiting in the medium, at which point the enzyme is formed in substantial amount at a maximum rate. The implications of the findings with respect to mechanisms of controlling enzyme dormation are discussed.