Relationships between conformational stabilities and surface functional properties of mutant hen egg-white lysozymes constructed by genetic engineering
Relationships between conformational stabilities and surface functional properties of mutant hen egg-white lysozymes constructed by genetic engineering
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基因工程构建的突变鸡蛋清溶菌酶构象稳定性与表面功能特性的关系
DOI：
10.1021/jf00037a041
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发表时间：
1994
影响因子：
6.1
通讯作者：
Kunihiko Kobayashi
中科院分区：
文献类型：
--
作者：
A. Kato;S. Tanimoto;Yoshifumi Muraki;Y. Oda;Y. Inoue;Kunihiko Kobayashi
引入基因工程方法来阐明鸡蛋清溶菌酶（HEWL）的构象稳定性和表面功能特性之间的关系。为了获得具有不同稳定性的突变体，通过定点诱变对HEWL的cDNA进行氨基酸替换，以去除Cys 76和Cys 94（C94A）之间的S-S键以及删除Lys13和Leu129（K13D）之间的盐键。将突变溶菌酶 C94A 和 K13D 的 cDNA 插入酵母表达载体 (PYG-100) 并在酿酒酵母中表达
A genetic engineering approach was introduced to elucidate the relationships between conformational stabilities and surface functional properties of hen egg-white lysozyme (HEWL). To obtain the mutants haring different stabilities, amino acid replacements were carried out by site-directed mutagenesis in the cDNA of HEWL to remove the S-S bond between Cys 76 and Cys 94 (C94A) and to delete the salt linkage between Lys13 and Leu129 (K13D). The cDNAs of mutant lysozymes C94A and K13D were inserted into a yeast expression vector (PYG-100) and expressed in Saccharomyces cerevisiae