Induction of cell growth arrest by atmospheric non-thermal plasma in colorectal cancer cells
Induction of cell growth arrest by atmospheric non-thermal plasma in colorectal cancer cells
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DOI：
10.1016/j.jbiotec.2010.10.003
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发表时间：
2010-12-01
影响因子：
4.1
通讯作者：
Baek, Seung Joon
中科院分区：
文献类型：
--
作者：
Kim, Chul-Ho;Bahn, Jae Hoon;Baek, Seung Joon
等离子体是由电离中性气体分子产生的，产生包括电子和离子在内的能量粒子的混合物。近年来对非热大气等离子体的认识取得了进展，并在生物医学上得到了应用。然而，血浆诱导细胞生长阻滞的确切分子机制尚不清楚。在这项研究中，我们探讨了非热大气等离子体治疗癌症的可行性，并探讨了等离子体诱导人类结直肠癌细胞抗增殖特性和细胞死亡的机制。非热大气等离子体诱导细胞生长停滞和诱导细胞凋亡。此外，血浆降低了细胞的迁移和侵袭活性。结果，我们发现对细胞的血浆处理增加了β -连环蛋白的磷酸化。提示-连环蛋白降解至少在部分血浆诱导的抗增殖活性中起作用。因此，非热大气等离子体构成了一种新的生物工具，具有调节细胞信号传导和功能的治疗应用潜力。(C) 2010 Elsevier B.V.版权所有
Plasma is generated by ionizing neutral gas molecules, resulting in a mixture of energy particles, including electrons and ions. Recent progress in the understanding of non-thermal atmospheric plasma has led to applications in biomedicine. However, the exact molecular mechanisms involved in plasma-induced cell growth arrest are unclear. In this study, we investigated the feasibility of non-thermal atmospheric plasma treatment for cancer therapy and examined the mechanism by which plasma induces anti-proliferative properties and cell death in human colorectal cancer cells. Non-thermal atmospheric plasma induced cell growth arrest and induced apoptosis. In addition, plasma reduced cell migration and invasion activities. As a result, we found that plasma treatment to the cells increases beta-catenin phosphorylation. suggesting that beta-catenin degradation plays a role at least in part in plasma-induced anti-proliferative activity. Therefore, non-thermal atmospheric plasma constitutes a new biologic tool with the potential for therapeutic applications that modulate cell signaling and function. (C) 2010 Elsevier B.V. All rights reserved.