Differential responses of G-protein Arabidopsis thaliana mutants to ozone
Differential responses of G-protein Arabidopsis thaliana mutants to ozone
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DOI：
10.1111/j.1469-8137.2004.01081.x
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发表时间：
2004-06-01
期刊：
影响因子：
9.4
通讯作者：
Jones, AM
中科院分区：
文献类型：
--
作者：
Booker, FL;Burkey, KO;Jones, AM
地面臭氧（O-3）减少了全世界许多地区的农业生产。然而，O-3毒性的病因仍不清楚。活性氧物种似乎造成生物化学病变和传播复合植物损伤的防御反应。由于一些植物防御反应涉及膜界定的GTP酶，（G蛋白），我们评估了异源三聚体G蛋白途径中改变的拟南芥突变体的O-3敏感性0、100、175和250 nmol mol mol（-1））处理13 d后，通常在野生型叶中观察到的上向性在缺乏G蛋白复合体α亚基（gpal）的突变体植物中不发生。O-3诱导的抑制叶片叶绿素水平和单位叶面积的叶质量的gpal突变体较少，并没有由于O-3 flux.There是一个G-蛋白α亚基的缺乏和O-3敏感性下降之间的正相关。我们的研究结果表明，异源三聚体G蛋白是至关重要的参与O-3的影响在植物中的表达。
Ground-level ozone (O-3) curtails agricultural production in many regions worldwide. However, the etiology of O-3 toxicity remains unclear. Activated oxygen species appear to inflict biochemical lesions and propagate defense responses that compound plant injury. Because some plant defense responses involve membrane-delimited GTPases (G proteins), we evaluated the O-3 sensitivity of Arabidopsis mutants altered in the heterotrimeric G-protein pathway.Eight genotypes were treated with a range of O-3 concentrations (0, 100, 175 and 250 nmol mol(-1)) for 13 d in controlled environment chambers.After treatment with O-3, the epinasty typically observed for wild type leaves did not occur in mutant plants lacking the alpha subunit of the G-protein complex (gpal). O-3-induced suppression of leaf chlorophyll levels and leaf mass per unit leaf area were less for gpal mutants and were not due to differences in O-3 flux.There was a positive correlation between the lack of a G-protein alpha subunit and decreased O-3 sensitivity. Our results suggest that a heterotrimeric G-protein is critically involved in the expression of O-3 effects in plants.