Met-enkephalin-Arg6-Gly7-Leu8-like immunoreactivity in mammalian taste buds.
Met-enkephalin-Arg6-Gly7-Leu8-like immunoreactivity in mammalian taste buds.
复制标题
哺乳动物味蕾中的 Met-脑啡肽-Arg6-Gly7-Leu8 样免疫反应性。
DOI：
10.1679/aohc.56.495
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发表时间：
1993
影响因子：
--
通讯作者：
T. Fujita
中科院分区：
文献类型：
--
作者：
S. Yoshie;C. Wakasugi;H. Kanazawa;T. Fujita
对大鼠、小鼠和豚鼠轮廓乳头的味蕾进行了各种生物活性肽（包括一些胃肠胰腺激素和神经元酶神经元特异性烯醇化酶（NSE））的光镜免疫细胞化学。在所有种属中，均显示Met-脑啡肽-Arg 6-Gly 7-Leu 8（Met-Enk-8）免疫反应阳性。Met-Enk-8样免疫反应仅限于部分梭形芽细胞。这些细胞在大鼠和豚鼠显示出伴随的NSE免疫反应性。然而，在小鼠的味蕾中，免疫反应之间似乎没有这种相关性。考虑到我们以前的研究结果，本研究结果支持了前脑啡肽原A衍生的阿片肽可能是味觉细胞的递质的可能性。
Light-microscopic immunocytochemistry for various bioactive peptides including some gastroenteropancreatic hormones and a neuronal enzyme, neuron-specific enolase (NSE), was applied to taste buds in the circumvallate papillae of rats, mice and guinea pigs. In all the species positive immunoreactivities were demonstrated for Met-enkephalin-Arg6-Gly7-Leu8 (Met-Enk-8). The Met-Enk-8-like immunoreactivity was confined to parts of the spindle-shaped bud cells. These cells in rats and guinea pigs displayed a concomitant immunoreactivity for NSE. In the mouse taste buds, however, there appeared no such correlation between the immunoreactivities. Considering our previous data, the present finding supports a possibility that opioid peptide(s) derived from preproenkephalin A might be the transmitter(s) of the gustatory cell.