wbeT sequence typing and IS1004 profiling of Vibrio cholerae isolates (Retracted article. See vol. 76, 2023)
wbeT sequence typing and IS1004 profiling of Vibrio cholerae isolates (Retracted article. See vol. 76, 2023)
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DOI：
10.1111/j.1472-765x.2012.03204.x
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发表时间：
2012-04-01
影响因子：
2.4
通讯作者：
Pourshafie, M. R.
中科院分区：
文献类型：
--
作者：
Sharifnia, A.;Bakhshi, B.;Pourshafie, M. R.
目的：调查霍乱弧菌O1血清型变异的分子基础和2004年至2008年在伊朗分离的不同血清型之间的遗传相关性。方法和结果：尽管存在所有三种血清型霍乱弧菌O1（小川，稻叶和Hikojima）在伊朗在过去的十年中，稻叶菌株已占主导地位的血清型。wbeT的序列分析确定只有一个单一的取代G为A的位置295在所有稻叶株导致丝氨酸脯氨酸的替代。在经典菌株和El Tor霍乱弧菌O1菌株之间未发现IS 1004的拷贝数和谱差异，这支持了在伊朗5年以上获得的分离株之间的克隆性。此外，我们的小川和稻叶分离株的HpaII消化的染色体DNA的Southern印迹显示存在的IS1004的不完整拷贝的所有solutions.Conclusions：IS1004分析可以是一个可靠的方法，用于分析的克隆传播的霍乱弧菌。结果表明，伊朗霍乱弧菌O1株wbeT内特定位置可能发生特异性点突变，进而可能导致血清型转换。研究的意义和影响：了解霍乱弧菌血清型转换的分子基础及其遗传相关性可以为即将到来的霍乱疫情预测和控制提供见解。
Aims: To investigate the molecular basis for serotype variation in Vibrio cholerae O1 and the genetic relatedness amongst different serotypes isolated from 2004 to 2008 in Iran.Methods and Results: Despite the presence of all three serotypes of V. cholerae O1 (Ogawa, Inaba and Hikojima) in Iran in the last decade, the Inaba strains have been the dominated serotype. Sequence analysis of wbeT determined only a single substitution of G for A at position 295 in all Inaba strains resulting in a replacement of serine to proline. No difference was found in the copy numbers and profile of IS1004 between the classical and El Tor V. cholerae O1 strains, supporting the clonality amongst the isolates obtained over 5 years in Iran. In addition, Southern blots of HpaII-digested chromosomal DNAs of our Ogawa and Inaba isolates showed the presence of an incomplete copy of IS1004 for all isolates.Conclusions: IS1004 profiling can be a reliable method for analysis of clonal dissemination of V. cholerae. The results indicated that specific point mutation at a particular position within the wbeT of V. cholerae O1 strains in Iran may occur which, in turn, may result in serotype switching. Significance and Impact of the Study: Understanding the molecular basis for serotype conversion of V. cholerae and their genetic relatedness could give insights for the incoming cholera epidemic prediction and control.