Pale green mutant analyses reveal the importance of CsGLKs in chloroplast developmental regulation and their effects on flavonoid biosynthesis in tea plant
Pale green mutant analyses reveal the importance of CsGLKs in chloroplast developmental regulation and their effects on flavonoid biosynthesis in tea plant
复制标题
淡绿色突变体分析揭示了 CsGLK 在叶绿体发育调控中的重要性及其对茶树类黄酮生物合成的影响
DOI：
10.1016/j.plaphy.2019.11.036
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发表时间：
2020-01-01
影响因子：
6.5
通讯作者：
Wang, Xinchao
中科院分区：
文献类型：
--
作者：
Hao, Xinyuan;Zhang, Weifu;Wang, Xinchao
叶色变异茶树品种因其独特的外观和风味，在茶叶生产和研究中受到越来越多的关注。然而，由于遗传转化的不可用性和高度复杂的基因组，这种变化背后的分子机制知之甚少。在这里，一个天然茶树突变体产生浅绿色分支（pgb）的发现和特点。超微结构和生化分析表明，pgb突变体叶片叶绿体结构缺陷，色素含量显著低于正常对照。对叶绿体发育相关基因的综合表达检测进一步表明，在pgb突变体中CsGLK的显著下调可能是导致叶绿体缺陷的原因。转录组分析和多酚类化合物的测定表明，茶树光合作用与次生代谢产物的合成密切相关。这些结果为阐明茶树叶绿体发育和叶色变化的机理提供了有用的信息。
Tea cultivars with leaf color variation have attracted increasing attention in tea production and research due to their unusual appearances and appealing flavors. However, the molecular mechanism underlying this variation is little known due to the unavailability of genetic transformation and a highly complex genome. Here, a natural tea plant mutant producing pale green branches (pgb) was discovered and characterized. Ultrastructural and biochemical analyses showed that the leaves of the pgb mutant had defective chloroplast structure and significantly lower pigment content than the normal control. Comprehensive expression detection of chloroplast-development-related genes further indicated that a significant downregulation of CsGLKs in the pgb mutant likely caused the chloroplast defect. Transcriptome analyses and polyphenolic compound determination highlighted a tight correlation between photosynthesis and secondary metabolite biosynthesis in tea plant. These results provide useful information illuminating the mechanism of chloroplast development and leaf color variation in tea plant.