Serotoninergic modulation of chloride homeostasis during maturation of the locomotor network in zebrafish
Serotoninergic modulation of chloride homeostasis during maturation of the locomotor network in zebrafish
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DOI：
10.1523/jneurosci.2017-05.2005
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发表时间：
2005-11-16
影响因子：
5.3
通讯作者：
Drapeau, P
中科院分区：
文献类型：
--
作者：
Brustein, E;Drapeau, P
在发育过程中，神经网络通过重要的功能变化，如自发活动的产生，去极化氯离子梯度的表达和神经调节的出现而发展。关于这些过程如何整合以产生成熟的行为，我们知之甚少。我们以前表明，在成熟的运动网络的斑马鱼，内源性5-羟色胺（5 HT）增加运动活动，减少不活动的间隔，而不影响积极的游泳期，在其他和更成熟的制剂的目标是5 HT。由于膜的性质是恒定的休息期间，我们在这里检查是否5 HT调节氯稳态。我们比较了阻断（向内）氯共转运与布美他尼的影响，5 HT及其拮抗剂的影响，无论是行为视频成像和细胞全细胞和短杆菌肽穿孔补丁记录。布美他尼模仿5 HT拮抗剂的作用，通过延长休息时间，而不影响游泳事件的性质（持续时间;频率;去极化程度）无论是行为上还是在虚构的游泳。此外，布美他尼和5-HT拮抗剂抑制去极化反应的幅度诱发的甘氨酸对脊髓神经元的河豚毒素的存在下，并短暂抑制甘氨酸虚构的游泳后测量的反应幅度。布美他尼的作用与5-HT的作用相反，并阻断了5-HT的作用。我们认为，在发展过程中，内源性5-HT调节氯稳态在静止的时间间隔，从而抵消了长期的静止期通常观察到的发展中的网络，使表达持续和行为相关的活动。
During development, neural networks progress through important functional changes such as the generation of spontaneous activity, the expression of a depolarizing chloride gradient, and the appearance of neuromodulation. Little is known about how these processes are integrated to yield mature behaviors. We showed previously that, during the maturation of the locomotor network of the zebrafish, endogenous serotonin (5HT) increased motor activity by reducing intervals of inactivity, without affecting the active swim periods that are the target of 5HT in other and more mature preparations. Because membrane properties were constant during the rest intervals, we examined here whether 5HT modulates chloride homeostasis. We compared the effects of blocking (inward) chloride cotransport with bumetanide to the effects of 5HT and its antagonists, both behaviorally by video imaging and cellularly by whole-cell and gramicidin-perforated patch recordings. Bumetanide mimicked the effects of 5HT antagonists, by prolonging rest intervals without affecting the properties of swim episodes (duration; frequency; extent of depolarization) either behaviorally or during fictive swimming. Furthermore, bumetanide and 5HT antagonists suppressed the amplitude of depolarizing responses evoked by ionophoresis of glycine onto spinal neurons in the presence of tetrodotoxin and transiently suppressed the amplitude of responses to glycine measured after fictive swimming. The effects of bumetanide contrasted with and occluded the effects of 5HT. We suggest that, during development, endogenous 5HT modulates chloride homeostasis during the quiescent intervals and thereby offsets the long periods of quiescence commonly observed in developing networks to allow expression of sustained and behaviorally relevant activity.