Effect of lotus seedpod oligomeric procyanidins on AGEs formation in simulated gastrointestinal tract and cytotoxicity in Caco-2 cells.
Effect of lotus seedpod oligomeric procyanidins on AGEs formation in simulated gastrointestinal tract and cytotoxicity in Caco-2 cells.
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DOI：
10.1039/d0fo03152f
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发表时间：
2021-04
期刊：
影响因子：
6.1
通讯作者：
Qian Wu;Kuoquan Zhao;Yuanyuan Chen;Yuanyuan Ouyang;Yingna Feng;Shuyi Li;Liang Zhang;Nianjie Feng
中科院分区：
文献类型：
--
作者：
Qian Wu;Kuoquan Zhao;Yuanyuan Chen;Yuanyuan Ouyang;Yingna Feng;Shuyi Li;Liang Zhang;Nianjie Feng
本研究探讨莲房低聚原花青素(LSOPC)及其主要单体儿茶素(CC)在胃肠道消化过程中对晚期糖基化终产物(AGEs)形成和Caco-2细胞毒性的影响。研究发现，LSOPC和CC有效地抑制了模拟胃肠道消化中AGEs的形成，保护Caco-2细胞免受AGEs的攻击。CC对AGEs形成的抑制作用明显优于LSOPC。此外，它们还能有效抑制消化酶活性、活性氧、RAGE-p38MAPK-NF-κB信号通路、炎症因子(肿瘤坏死因子α、白介素6)和黏附因子(细胞间黏附分子-1、血管细胞黏附分子-1)，从而保护Caco-2细胞。
This study explored the effects of lotus seedpod oligomeric procyanidins (LSOPC) and their main monomer catechin (CC) on the formation of advanced glycation end products (AGEs) and Caco-2 cytotoxicity during gastrointestinal digestion. Studies have found that LSOPC and CC inhibited the AGEs formation effectively in simulated gastrointestinal digestion and protected Caco-2 cells from AGEs attack. The effect of CC on the inhibition of AGEs formation was significantly better than that of LSOPC. Further, they could effectively inhibit the digestive enzyme activity, reactive oxygen species, RAGE-p38MAPK-NF-κB signaling pathway, inflammatory factors (tumor necrosis factor alpha, interleukin 6), and adhesion factors (intercellular cell adhesion molecule-1, vascular cell adhesion molecule-1) to protect Caco-2 cells.