Investigation of Quercetin interaction behaviors with lipid bilayers: Toward understanding its antioxidative effect within biomembrane
Investigation of Quercetin interaction behaviors with lipid bilayers: Toward understanding its antioxidative effect within biomembrane
复制标题
DOI：
10.1016/j.jbiosc.2021.03.004
复制
发表时间：
2021-06-16
影响因子：
2.8
通讯作者：
Umakoshi, Hiroshi
中科院分区：
文献类型：
--
作者：
Han, Jin;Amau, Misaki;Umakoshi, Hiroshi
槲皮素（Quercetin，QCT）是一种含量丰富的平面结构生物活性物质，具有多种药理作用。本文制备了四种脂质体作为模型生物膜，评价其分配系数、在脂膜中的分布以及QCT对膜性质的影响。QCT在脂膜上的分配受膜相状态和QCT对膜性质的干扰两方面的影响。QCT在脂膜中的位置与脂膜的相态有关。此外，QCT对脂膜中烃尾压缩的影响取决于脂质分子的不饱和度。最后，在抗氧化活性方面，从2，2-二苯基-1-苦肼基自由基清除实验结果可以看出，QCT与脂膜的相互作用对QCT在脂膜中的还原活性有很大影响。此外，DOPC分子的质谱显示在QCT存在下没有脂质氧化，表明除了QCT对自由基的清除能力之外，QCT在不饱和脂质膜中的有序化效应将有助于通过抑制自由基扩散来保护脂质膜免受氧化（协同效应）。本研究在脂膜分析的基础上，明确了QCT对各种脂膜的影响及其与QCT在脂膜内抗氧化作用的关系。因此，我们的分析方法和发现也有助于了解其他抗氧化剂对生物膜作用的机制。(C)2021年，生物技术学会，日本。All rights reserved.
Quercetin (QCT), existing in common dietary sources, is an abundant bioflavonoid with planar structure and exerts multiple pharmacological effects. Herein, four kinds of liposomes were prepared as model biomembranes, and then the partition coefficient, distribution in lipid membrane and influence of the QCT on the membrane properties were evaluated. The partition of QCT to lipid membranes was affected by both membrane phase state and the interference of QCT on membrane properties. The location of QCT in lipid membrane was related to the phase state of lipid membrane. In addition, influence of QCT on the compaction of the hydrocarbon tail in lipid membranes was dependent on the unsaturation degree of lipid molecules. Finally, about its antioxidant activity, from the results of 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, it can be concluded that the interaction of QCT with lipid membrane greatly influences on QCT reductive activity in lipid membrane. Furthermore, mass spectrometry of DOPC molecule showed no lipid oxidation in the presence of QCT, indicating that in addition to the QCT ability toward radical scavenging, the ordering effect of QCT in unsaturated lipid membrane would be helpful to protect lipid membrane from oxidation by inhibiting radical diffusion (synergy effect). Based on lipid membrane analysis, our study made it clear that the effect of QCT on various lipid membrane and its relation with the antioxidant effect of QCT within lipid membrane. Therefore, our analytical method and findings would be also helpful for understanding the mechanism of other antioxidants effects on biomembrane. (C) 2021, The Society for Biotechnology, Japan. All rights reserved.