Immunocytochemical localization of a neuron-specific thrombospondin-1-like protein, NELL2: light and electron microscopic studies in the rat brain
Immunocytochemical localization of a neuron-specific thrombospondin-1-like protein, NELL2: light and electron microscopic studies in the rat brain
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DOI：
10.1016/s0169-328x(99)00342-3
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发表时间：
2000-03-10
期刊：
影响因子：
--
通讯作者：
Saito, N
中科院分区：
文献类型：
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作者：
Oyasu, M;Kuroda, S;Saito, N
我们已经研究了NELL 2，一种神经血小板反应蛋白-1-样蛋白的细胞和细胞内定位。使用针对NELL 2的C-末端区域产生的特异性抗体，NELL 2蛋白被检测为140和90 kDa的双重条带，并且仅在脑中被识别，而在外周组织中不被识别。在脑内，NELL 2大量存在于海马和大脑皮质中，在嗅球和下丘脑中中度存在，并且在丘脑、小脑和髓质中以低水平存在。免疫细胞化学，NELL 2仅见于神经元中，而不见于胶质细胞或白色物质中。NELL 2免疫反应阳性细胞分布于整个脑中，在海马和大脑皮质中密度最高。NELL 2主要分布于神经元胞体内，胞体周围呈点状分布。NELL 2免疫反应性的分布并不完全对应于蛋白激酶C（PKC）的任何亚型。电镜下，NELL 2蛋白与内质网（ER），尤其是粗面内质网结合。NELL 2免疫反应性被发现在ER的限制性部分，并发现通常在ER内。这些结果表明，NELL 2蛋白是由神经元合成的，并可能从参与某些神经元功能的神经元分泌。(C)2000 Elsevier Science B. V.保留所有权利。
We have studied the cellular and intracellular localization of NELL2, a neural thrombospondin-1-like protein. NELL2 protein was detected as doublet bands of 140 and 90 kDa with the use of the specific antibodies raised against the C-terminal region of NELL2 and was recognized only in the brain but not in the peripheral tissues. Within the brain, NELL2 was abundantly present in the hippocampus and cerebral cortex, found moderately in the olfactory bulb and hypothalamus, and at a low level in the thalamus, cerebellum, and medulla, Immunocytochemically, NELL2 was seen only in neurons but not in glial cells or in the white matter. NELL2-immunoreactive cells were distributed throughout the brain with the highest density in the hippocampus and cerebral cortex. NELL2 was mainly found in the cell bodies of neurons and the immunoreactivity was often seen as dots in the perikarya. The distribution of NELL2 immunoreactivity did not completely correspond to that of any subtypes of protein kinase C (PKC). Under electron microscopy, NELL2 protein was associated with the endoplasmic reticulum (ER), especially with rough ER. NELL2 immunoreactivity was found in the restricted parts of the ER and found commonly inside the ER. These results suggest that NELL2 protein is synthesized by neurons and may be secreted from the neurons involved in certain neuronal functions. (C) 2000 Elsevier Science B.V. All rights reserved.