Characterization of CsSEF1 gene encoding putative CCCH-type zinc finger protein expressed during cucumber somatic embryogenesis
Characterization of CsSEF1 gene encoding putative CCCH-type zinc finger protein expressed during cucumber somatic embryogenesis
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DOI：
10.1016/j.jplph.2008.06.005
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Filipecki, Marcin
中科院分区：
文献类型：
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作者：
Grabowska, Agnieszka;Wisniewska, Anita;Filipecki, Marcin
离体培养获得的体细胞胚是营养繁殖的一种形式，可用于人工种子技术，也可作为模式。研究植物生长的原理。为了分离黄瓜（Cucumis sativus L.）体细胞胚胎发生相关基因，采用抑制性消减杂交（SSH）技术。获得的序列之一是CsSEF1克隆（黄瓜体细胞胚胎发生锌指1），具有水平。随着胚胎发生的诱导，表达量急剧增加。CsSEF1的全长cDNA编码假定的307个氨基酸长的蛋白质，含有三个锌指基序，两个CCCH和一个非典型CHCH模式。CsSEF1蛋白与植物中的其他蛋白具有显著的相似性，其中锌指的排列和模式非常相似。CsSEF1的转录本位于体细胞胚的顶端部分，早在极性可见时就开始，在后期发育阶段标记子叶原基和原形成层组织。作为一个结果，转移到拟南芥的反义片段CsSEF1合子胚异常，子叶和根的发育进行了观察。(C)2008年Elsevier GmbH。All rights reserved.
Somatic embryos obtained in vitro are a form of vegetative reproduction that can be used in artificial seed technology, as welt as a model. to study the principles of plant development. In order to isolate the genes involved in somatic embryogenesis of the cucumber (Cucumis sativus L.), we utilized the suppression subtractive hybridization (SSH). One of the obtained sequences was the CsSEF1 clone (Cucumis sativus Somatic Embryogenesis Zinc Finger 1), with a level. of expression that sharply increased with the induction of embryogenesis. The full length cDNA of CsSEF1 encodes the putative 307 amino acid long protein containing three zinc finger motifs, two with CCCH and one with the atypical CHCH pattern. The CsSEF1 protein shows significant similarity to other proteins from plants, in which the zinc fingers arrangement and patterns are very similar. Transcripts of CsSEF1 were localized in the apical part of somatic embryos, starting as early as the polarity was visible and in later developmental stages marking the cotyledon primordia and procambium tissues. As a result of transferring an antisense fragment of CsSEF1 into Arabidopsis thaliana abnormalities in zygotic embryos and also in cotyledons and root development were observed. (C) 2008 Elsevier GmbH. All rights reserved.