Expression of Aquaglyceroporins in Epithelial Ovarian Tumours and Their Clinical Significance
Expression of Aquaglyceroporins in Epithelial Ovarian Tumours and Their Clinical Significance
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水甘油孔蛋白在上皮性卵巢肿瘤中的表达及其临床意义
DOI：
10.1177/147323001103900302
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发表时间：
2011-05-01
影响因子：
1.6
通讯作者：
Zhu, Y. S.
中科院分区：
文献类型：
--
作者：
Yang, J. H.;Yan, C. X.;Zhu, Y. S.
采用免疫组织化学和免疫印迹法检测了98例卵巢上皮性肿瘤组织和20例子宫肌瘤患者卵巢正常组织中AQP3、AQP7和AQP9的蛋白水平。免疫组织化学检测到AQP3和AQP9蛋白存在于良性和交界性肿瘤细胞的基底外膜中，分布于恶性肿瘤细胞的质膜中。AQP7蛋白定位于良性肿瘤细胞的质膜，但在交界性和恶性肿瘤细胞中，它选择性地染色在核膜上。Western blot结果显示，AQP7和AQP9蛋白在恶性和交界性肿瘤中的表达明显高于良性肿瘤和正常卵巢组织。AQP9表达水平与肿瘤分级、组织学分型呈正相关。由此可见，高水平的水甘油脂素表达可能是卵巢癌发生的重要因素。
Protein levels of aquaglyceroporins AQP3, AQP7 and AQP9 were measured by immunohistochemistry and Western blotting in epithelial ovarian tumour tissue from 98 patients and in normal ovary tissue from 20 persons with uterine myoma. AQP3 and AQP9 proteins were detected immunohistochemically in the basolateral membranes of benign and borderline tumour cells and were found to be distributed throughout the plasma membranes of malignant tumour cells. AQP7 protein was localized in the plasma membranes of benign tumour cells but, in borderline and malignant tumour cells, it was selectively stained in the nuclear membrane. Western blotting showed significantly higher AQP7 and AQP9 protein expression in malignant and borderline tumours than in benign tumour and normal ovarian tissue. AQP9 expression level was positively and significantly correlated with tumour grade and histological type. It was concluded that a high level of aquaglyceroporin expression may be an important factor in ovarian carcinogenesis.