Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails -: art. no. R17
Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails -: art. no. R17
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DOI：
10.1186/gb-2005-6-2-r17
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发表时间：
2005-01-01
期刊：
影响因子：
12.3
通讯作者：
Iino, Y
中科院分区：
文献类型：
--
作者：
Kunitomo, H;Uesugi, H;Iino, Y
将微阵列技术应用于少量细胞并不总是容易的，因为难以从这些细胞中选择性地分离mRNA。我们在这里报告的mRNA制备的纤毛感觉神经元的秀丽隐杆线虫使用mRNA标记的方法，其中聚（A）RNA与抗原表位标记的聚（A）结合蛋白特异性表达的感觉神经元免疫共沉淀。随后的cDNA微阵列分析导致了一组感觉神经元表达基因的鉴定。
It is not always easy to apply microarray technology to small numbers of cells because of the difficulty in selectively isolating mRNA from such cells. We report here the preparation of mRNA from ciliated sensory neurons of Caenorhabditis elegans using the mRNA-tagging method, in which poly( A) RNA was co-immunoprecipitated with an epitope-tagged poly(A)-binding protein specifically expressed in sensory neurons. Subsequent cDNA microarray analyses led to the identification of a panel of sensory neuron-expressed genes.