Biogeochemical activity of anaerobic microorganisms from buried permafrost sediments
Biogeochemical activity of anaerobic microorganisms from buried permafrost sediments
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DOI：
10.1080/01490459809378075
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发表时间：
1998-07-01
影响因子：
2.3
通讯作者：
McGrath, J
中科院分区：
文献类型：
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作者：
Rivkina, E;Gilichinsky, D;McGrath, J
从俄罗斯北极东北部地区的多年冻土沉积物样品，年龄从7000年到200万年不等，进行了分析，以获得还原条件，厌氧细菌及其代谢终产物的可行种群的证据。对样品的实地分析表明，所有沉积物都被还原，氧化还原电位在+40 mV至-256 mV之间。在冻结的沉积物中检测到亚铁、酸溶性硫化物和甲烷。直接细菌计数是！使用吖啶橙子通过落射荧光显微镜测定的10（7）至10（8）个细胞/g沉淀物。反硝化菌和（H-2 + CO2）-利用产甲烷菌在所有样品中检测到，和acetoclastic产甲烷菌，硫酸盐还原剂，和Fe（III）还原剂在一些样品中检测到，在低得多的数字。在4 ℃厌氧培养6个月的土壤中检测到[S-35]硫酸盐产生的[S-35]硫化物。因此，在接近冰点的温度下存在厌氧代谢活动。这些结果表明，生活厌氧细菌居住在老化，减少永冻层沉积物。未来的研究应侧重于检测冻结状态下的活动。
Permafrost sediment samples, ranging in age from 7 thousand to 2 million years, from the northeastern region of Russian Arctic were analyzed for evidence of reducing conditions, viable populations of anaerobic bacteria and their metabolic endproducts. Field analyses of samples showed that all sediments were reduced with a redox potential ranging from +40 to -256 mV. Ferrous iron, acid-soluble sulfide, and methane were detected in the frozen sediments. Direct bacterial counts were! 10(7) to 10(8) cells/g sediments as determined by epifluorescence microscopy using acridine orange. Denitrifiers and (H-2 + CO2)-utilizing methanogens were detected in all samples, and acetoclastic methanogens, sulfate reducers, and Fe(III) reducers were detected in some samples and at much lower numbers. [S-35]Sulfide production from [S-35]sulfate was detected in soils incubated anaerobically at 4 degrees C for 6 months. Thus anaerobic metabolic activity was present at temperatures near freezing. These results suggest that viable anaerobic bacteria reside in aged, reduced permafrost sediments. Future investigation should focus on detecting activity in the frozen state.