Detection of Norovirus and Rotavirus Present in Suspended and Dissolved Forms in Drinking Water Sources
Detection of Norovirus and Rotavirus Present in Suspended and Dissolved Forms in Drinking Water Sources
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DOI：
10.1007/s12560-018-9361-5
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发表时间：
2019-03-01
影响因子：
3.4
通讯作者：
Akiba, Michihiro
中科院分区：
文献类型：
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作者：
Miura, Takayuki;Gima, Arisa;Akiba, Michihiro
我们调查了饮用水生产用地表水中第二组诺沃克病毒和A组轮状病毒的存在形式。从2017年6月至2018年8月，每月从饮用水处理厂(DWTP)采集河水样本(N=15)，通过10亩和0.45亩孔径的膜过滤进行分级，并定量检测以悬浮和溶解形式存在的病毒。诺如病毒GII存在于10亩和0.45亩的悬浮物和溶解物中，检出率分别为33%、60%和87%。轮状病毒A在各种形态中的检出频率均高于诺如病毒GII(10微米悬液，73%；0.45-10微米悬液，93%；溶解，100%)。在胃肠炎非流行季节和流行季节，我们还对日本各地21个污水处理厂的地表水样本进行了分析。在非流行季节和流行季节，分别有48%和81%的样本以溶解形式检测到诺沃克病毒GII，其浓度分别高达4.1log(10)拷贝/L和5.3log(10)拷贝/L，GII主要是2012年悉尼的基因，其次是GII。甲型轮状病毒在不同季节分别有95%和86%的样品以溶解形式存在，最高浓度分别为5.5和6.3log(10)拷贝/L。诺如病毒GII浓度在0.45-10微米悬浮液和溶解液中相近，而对轮状病毒A有显著差异(P
We investigated the present forms of genogroup II norovirus and group A rotavirus in surface water used for drinking water production. River water samples (N=15) collected at a drinking water treatment plant (DWTP) monthly from June 2017 to August 2018 were fractioned by filtration through 10- and 0.45-mu m-pore-size membranes, and viruses present in suspended and dissolved forms were quantitatively detected. Norovirus GII was present in >10-mu m- and 0.45-10-mu m-suspended and dissolved forms with detection rates of 33%, 60%, and 87%, respectively. Rotavirus A was detected more frequently than norovirus GII in each form (>10 mu m suspended, 73%; 0.45-10 mu m suspended, 93%; dissolved, 100%). We also analyzed surface water samples from 21 DWTPs all over Japan in non-epidemic and epidemic seasons of gastroenteritis. Norovirus GII was detected in 48% and 81% of samples with the concentrations of up to 4.1 and 5.3 log(10) copies/L in dissolved form in non-epidemic and epidemic seasons, respectively, and GII.4 Sydney 2012 was predominant genotype followed by GII.2. Rotavirus A was detected in 95% and 86% of samples with the maximum concentrations of 5.5 and 6.3 log(10) copies/L in dissolved form in respective seasons. Concentration of norovirus GII was similar in 0.45-10-mu m suspended and dissolved forms, while there was a significant difference for rotavirus A (P