Identification and differentiation of Legionella pneumophila and Legionella spp. with real-time PCR targeting the 16S rRNA gene and species identification by mip sequencing
Identification and differentiation of Legionella pneumophila and Legionella spp. with real-time PCR targeting the 16S rRNA gene and species identification by mip sequencing
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DOI：
10.1128/aem.02839-05
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发表时间：
2006-09-01
影响因子：
4.4
通讯作者：
Bergh, Kare
中科院分区：
文献类型：
--
作者：
Stolhaug, Anne;Bergh, Kare
构建了针对16S rRNA基因的荧光共振能量转移探针，构建了一种灵敏特异的实时PCR方法，用于嗜肺军团菌与其他军团菌的鉴别和分化。通过直接扩增子测序，建立了两种针对inip基因的常规PCR检测方法。
Fluorescent resonance energy transfer probes targeting the 16S rRNA gene were constructed for a sensitive and specific real-time PCR for identification and differentiation of Legionella pneumophila from other Legionella spp. For identification of non-L.pneumophila spp. by direct amplicon sequencing, two conventional PCR assays targeting the inip gene were established.