Epithelial Differentiation of Adipose-Derived Stem Cells for Laryngeal Tissue Engineering
Epithelial Differentiation of Adipose-Derived Stem Cells for Laryngeal Tissue Engineering
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DOI：
10.1002/lary.20719
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发表时间：
2010-01-01
期刊：
影响因子：
2.6
通讯作者：
Chhetri, Dinesh K.
中科院分区：
文献类型：
--
作者：
Long, Jennifer L.;Zuk, Patricia;Chhetri, Dinesh K.
目标/假设：一种潜在的治疗选择。严重的声带疤痕是用含有自体细胞的组织工程结构取代声带覆盖层。作为实现这一目标的第一步，我们寻求开发一种类似于固有层和上皮声带层的三维细胞填充基质。研究设计：基础科学研究。方法：在含有各种生长因子的纤维蛋白水凝胶中培养脂肪干细胞。在培养期结束时，将基质切片并用免疫标记物标记以鉴定细胞表型。结果：脂肪干细胞存活、附着并填充三维纤维蛋白基质。在选定的条件下，表达上皮标记蛋白的细胞表层覆盖更深的间充质细胞层。 结论：创建了纤维蛋白和脂肪干细胞的三维结构作为声带替代物的原型。出现了两种分离的细胞表型，在固有层上产生类似于上皮的双层结构。这项初步工作证明了组织工程生产声带置换结构的可行性。
Objectives/Hypothesis: One potential treatment option for. severe Vocal fold scarring is to replace the vocal fold cover layer with a tissue-engineered structure containing autologous cells. As a first step toward that goal, we sought to develop a three-dimensional cell-populated matrix resembling the vocal fold layers of lamina propria and epithelium.Study Design: Basic science investigation.Methods: Adipose-derived stem cells were cultured in fibrin hydrogels' with various, growth factors. At the end of the culture period, matrices were sectioned and labeled with immunomarkers to identify cell phenotype.Results: Adipose-derived stem cells survived, attached, and populated three-dimensional fibrin matrices. Under select conditions, a superficial layer of cells expressing, epithelial marker proteins overlay a deeper mesenchymal cell layer.Conclusions: A three-dimensional structure of fibrin and adipose-derived stem cells was created as a prototype vocal fold replacement. Two segregated cell phenotypes occurred, producing a bilayered structure resembling epithelium over lamina propria. This preliminary work demonstrates, I the feasibility of tissue engineering to produce structures for vocal fold replacement.