Effect of anabolic steroids on skeletal muscle mass during hindlimb suspension.
Effect of anabolic steroids on skeletal muscle mass during hindlimb suspension.
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DOI：
10.1152/jappl.1987.63.5.2122
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发表时间：
1987-11
影响因子：
3.3
通讯作者：
R. Tsika;R. Herrick;K. Baldwin
中科院分区：
文献类型：
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作者：
R. Tsika;R. Herrick;K. Baldwin
合成代谢类固醇治疗[每天 0.3 或 0.9 毫克癸酸诺龙 (Deca-Durabolin)] 的功效在不影响啮齿动物快肌跖肌和慢肌比目鱼肌重量、不影响亚细胞蛋白以及改变后肢悬浮反应的异肌球蛋白表达的背景下进行了检查。将雌性大鼠分为四组（7只大鼠/组，持续6周）：1）正常对照（NC），2）正常类固醇（NS），3）正常悬浮液（N-SUS）和4）悬浮类固醇（SUS-S）。与跖肌和比目鱼肌的对照值相比，悬浮引起 1) 体重和肌肉重量减小（P 小于 0.05），2) 肌原纤维含量减少（mg/肌肉，P 小于 0.05），3) 异肌球蛋白相对表达（表示为总异肌球蛋白的百分比）发生变化，有利于较少的慢肌球蛋白和较大的快肌球蛋白同种型（P 小于0.05）。悬浮动物的类固醇治疗（SUS-S 与 N-SUS）部分减轻了身体和肌肉重量（P 小于 0.05）和跖肌的影响，但不影响比目鱼肌肌原纤维含量（毫克/肌肉，P 小于 0.05）。然而，类固醇治疗并没有改变悬浮诱导的异肌球蛋白模式。在正常大鼠（NS 与 NC）中，类固醇治疗增加了身体和跖肌重量，但没有增加比目鱼肌重量（P 小于 0.05），并且不会改变任一肌肉类型中的异肌球蛋白表达。总的来说，这些数据表明，在年轻雌性大鼠中，合成代谢类固醇 1) 增强正常大鼠的体重和快肌踝伸肌的重量，2) 改善与后肢悬吊相关的体重、快肌重量和蛋白质含量以及慢肌重量的损失。（摘要截断为 250 字）
The efficacy of anabolic steroid treatment [0.3 or 0.9 mg nandrolone decanoate (Deca-Durabolin) per day] was examined in the context of sparing rodent fast-twitch plantaris and slow-twitch soleus muscle weight, sparing subcellular protein, and altering isomyosin expression in response to hindlimb suspension. Female rats were assigned to four groups (7 rats/group for 6 wk): 1) normal control (NC), 2) normal steroid (NS), 3) normal suspension (N-SUS), and 4) suspension steroid (SUS-S). Compared with control values for the plantaris and soleus muscles, suspension induced 1) smaller body and muscle weight (P less than 0.05), 2) losses in myofibril content (mg/muscle, P less than 0.05), and 3) shifts in the relative expression (expressed as %of total isomyosin) of isomyosins which favored lesser slow myosin and greater fast myosin isotypes (P less than 0.05). Steroid treatment of suspended animals (SUS-S vs. N-SUS) partially spared body and muscle weight (P less than 0.05) and spared plantaris but not soleus myofibril content (mg/muscle, P less than 0.05). However, steroid treatment did not modify the isomyosin pattern induced by suspension. In normal rats (NS vs. NC), steroid treatment enhanced body and plantaris muscle weight but not soleus weight (P less than 0.05) and did not alter isomyosin expression in either muscle type. Collectively these data suggest that in young female rats anabolic steroids 1) enhance the body weight and the weight of a fast-twitch ankle extensor in normal rats, 2) ameliorate the loss in body weight, fast-twitch muscle weight and protein content and slow-twitch muscle weight associated with hindlimb suspension.(ABSTRACT TRUNCATED AT 250 WORDS)