Genetic studies to re-affiliate Edwardsiella tarda fish isolates to Edwardsiella piscicida and Edwardsiella anguillarum species
Genetic studies to re-affiliate Edwardsiella tarda fish isolates to Edwardsiella piscicida and Edwardsiella anguillarum species
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DOI：
10.1016/j.syapm.2017.09.004
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发表时间：
2018-01-01
影响因子：
3.4
通讯作者：
Magarinos, Beatriz
中科院分区：
文献类型：
--
作者：
Bujan, Noemi;Mohammed, Haitham;Magarinos, Beatriz
在2012年之前，爱德华氏菌属由迟缓爱德华氏菌（Edwardsiella tarda）、保志爱德华氏菌（Edwardsiella hoshinae）和叉尾爱德华氏菌（Edwardsiella ictaluri）三个种组成。2013年，杀鱼爱德华氏菌（Edwardsiella piscicida），编译了以前鉴定为E. tarda，最近又报道了从病鳗中分离到的一个新种，即鳗爱德华氏菌（Edwardsiellaanguillarum），将这些种归入该属，使得以前鉴定为鳗爱德华氏菌的分离物的分类地位有必要作一修正。迟到。利用AFLP技术、MLSA技术和电子计算机DNA-DNA杂交技术，对49株E.迟发型E. piscicida和E. anguillarum，而证实了以前的爱德华氏菌类型和参考菌株的分类。对16 SrRNA、adk、atpD、dnal ginA、hsp 60、tuf等基因的分类学分辨以及管家基因之间可能的组合进行了研究，结果表明，dnaJ基因的分类学分辨力较高。总之，分子生物学技术的应用对于准确鉴定爱德华氏菌是必要的，特别是在区分新种和E。迟到。(C)2017 Elsevier GmbH. All rights reserved.
Until 2012, the genus Edwardsiella was composed by three species Edwardsiella tarda, Edwardsiella hoshinae and Edwardsiella ictaluri. In 2013, Edwardsiella piscicida, compiling fish pathogenic strains previously identified as E. tarda was described, and more recently a new species isolated from diseased eel was reported, namely Edwardsiella anguillarum.The incorporation of these species into the genus makes necessary a revision of the taxonomic position of the isolates previously identified as E. tarda. Using AFLP technique, MLSA studies and in silico DNA-DNA hybridization, 46 of 49 E. tarda isolates were re-assigned as E. piscicida and 2 as E. anguillarum, whereas it was confirmed previous classification of the Edwardsiella types and reference strains used. The study of the taxonomic resolution of the genes 16S rRNA, adk, atpD, dnal ginA, hsp60, tuf as well as the possible combinations among housekeeping genes, showed that the gene dnaJ was the more resolutive. In conclusion, the use of molecular techniques is necessary to accurately identify Edwardsiella isolates, especially when differentiating new species from E. tarda. (C) 2017 Elsevier GmbH. All rights reserved.