Effect of Bifidobacterium longum ingestion on experimental salmonellosis in mice
Effect of Bifidobacterium longum ingestion on experimental salmonellosis in mice
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DOI：
10.1111/j.1365-2672.2004.02265.x
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发表时间：
2004-01-01
影响因子：
4
通讯作者：
Nicoli, JR
中科院分区：
文献类型：
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作者：
Silva, AM;Barbosa, FHF;Nicoli, JR
目的：乳酸菌对免疫系统的影响在正常条件下和通常通过体内测定得到了很好的确立，但在感染性挑战期间，体内数据很少。本研究的目的是获得数据的假定的保护作用，双歧杆菌后的挑战与肠道pathogene.Methods和结果：口服治疗的效果与双歧杆菌长Bb 46胃内挑战鼠伤寒沙门氏菌进行了研究。在无菌（GN）小鼠中测定粪便细菌水平，在常规（CV）小鼠中测定死亡率、组织病理学（肠、肝）、免疫球蛋白水平（IgM、IgG、IgG 1、IgG 2a）和细胞因子产生（IFN-γ、IL-10）。常规小鼠在口服病原体攻击（10（2）CFU）前10天每天接受0.1ml益生菌乳（10（8）CFU）。然后，继续益生菌处理直到实验结束。无菌小鼠中的益生菌治疗由实验开始时的单剂量组成。对照组用无菌脱脂牛奶处理，并进行相同的程序。与对照组（0%）相比，观察到益生菌处理的动物的存活率更高（40%）。组织病理学和形态学数据证实了这种保护作用。然而，S.粪便中的鼠伤寒菌群水平在对照组和益生菌处理组之间相似。在挑战S。鼠伤寒沙门氏菌，IFN-γ和IgG 2a生产的减少，观察益生菌治疗mice.Conclusions：对病原体的挑战的保护作用可能是由于减少炎症反应，介导的益生菌治疗，但不是人口antagonistics.Significance和影响的研究：结果表明，饮食补充B。Longum可以提供对抗肠道感染的益处。
Aims: The effect of lactic acid bacteria on the immune system is well established under normal conditions and generally by in vivo determinations, but few data are available, in vivo, during an infectious challenge. The objective of this study was to obtain data on the putative protective role of bifidobacteria upon challenge with an intestinal pathogen.Methods and Results: The effect of oral treatment with Bifidobacterium longum Bb46 on intragastric challenge with Salmonella Typhimurium was studied. Faecal bacterial levels were determined in gnotobiotic (GN) mice and mortality, histopathology (intestines, liver), immunoglobulin levels (IgM, IgG, IgG1, IgG2a) and cytokine production (IFN-gamma, IL-10) were determined in conventional (CV) mice. Conventional mice received 0.1 ml probiotic milk (10(8) CFU) daily, 10 days before the oral pathogenic challenge (10(2) CFU). Then, probiotic treatment was continued until the end of the experiment. Probiotic treatment in germ-free mice consisted of a single dose at the beginning of the experiment. Control groups were treated with sterile skim milk and submitted to the same procedure. A higher survival (40%) was observed for probiotic-treated animals when compared with the control group (0%). This protective effect was confirmed by the histopathological and morphometric data. However, S. Typhimurium population levels in the faeces were similar among control and probiotic-treated groups. During the challenge with S. Typhimurium, a decrease in IFN-gamma and IgG2a productions was observed in probiotic-treated mice.Conclusions: The protective effect against the pathogenic challenge may be due to a reduced inflammatory response, mediated by the probiotic treatment, but not to a population antagonism.Significance and Impact of the Study: Results suggest that dietary supplementation with B. longum could provide benefits against enteric infection.