Haplotype analysis of vernalization loci in European barley germplasm reveals novel VRN-H1 alleles and a predominant winter VRN-H1/VRN-H2 multi-locus haplotype
Haplotype analysis of vernalization loci in European barley germplasm reveals novel VRN-H1 alleles and a predominant winter VRN-H1/VRN-H2 multi-locus haplotype
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DOI：
10.1007/s00122-007-0626-x
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发表时间：
2007-11-01
影响因子：
5.4
通讯作者：
O'Sullivan, Donal M.
中科院分区：
文献类型：
--
作者：
Cockram, James;Chiapparino, Elena;O'Sullivan, Donal M.
在大麦中，春化（开花前的一段较长的低温期）要求的变化由VRN-H1、-H2和-H3基因座决定。在欧洲栽培种质中，春化要求的大多数变异仅由VRN-H1和VRN-H2的等位基因引起，但等位基因变异的范围在很大程度上未被探索。在这里，我们将VRN-H1和VRN-H2单倍型在429个品种代表了大部分种植面积的大麦在西欧在过去的60年。分析基因型，内含子I测序数据和生长习性测试确定了三个新的VRN-H1等位基因，并确定了最常见的VRN-H1内含子I重排。联合分析VRN-H1和VRN-H2等位基因，得到17个VRN-H1/VRN-H2多位点单倍型，其中3个单倍型占品种总数的79%。这里采用的分子标记是预测生长习性和品种纯度评估的有力诊断工具。这些标记还将允许开发种质以测试单个等位基因的行为，目的是了解等位基因变异与对特定农业环境的适应之间的关系。
In barley, variation in the requirement for vernalization (an extended period of low temperature before flowering can occur) is determined by the VRN-H1, -H2 and -H3 loci. In European cultivated germplasm, most variation in vernalization requirement is accounted for by alleles at VRN-H1 and VRN-H2 only, but the range of allelic variation is largely unexplored. Here we characterise VRN-H1 and VRN-H2 haplotypes in 429 varieties representing a large portion of the acreage sown to barley in Western Europe over the last 60 years. Analysis of genotype, intron I sequencing data and growth habit tests identified three novel VRN-H1 alleles and determined the most frequent VRN-H1 intron I rearrangements. Combined analysis of VRN-H1 and VRN-H2 alleles resulted in the classification of seventeen VRN-H1/VRN-H2 multi-locus haplotypes, three of which account for 79% of varieties. The molecular markers employed here represent powerful diagnostic tools for prediction of growth habit and assessment of varietal purity. These markers will also allow development of germplasm to test the behaviour of individual alleles with the aim of understanding the relationship between allelic variation and adaptation to specific agri-environments.