Distribution of active and inactive forms of endorphins in rat pituitary and brain.
Distribution of active and inactive forms of endorphins in rat pituitary and brain.
复制标题
大鼠垂体和大脑中活性和非活性形式的内啡肽的分布。
DOI：
--
复制
发表时间：
1979
影响因子：
11.1
通讯作者：
D. Smyth
中科院分区：
文献类型：
--
作者：
S. Zakarian;D. Smyth
最近分离和鉴定了α-N-乙酰基形式的促脂素C片段（β-内啡肽，残基61-91）和C ′-片段（残基61-87）[Smyth，D.G.，Massey，D. E.，Zakarian，S. & Finnie，M.（1979）Nature（伦敦）279，252-254]已经导致了它们在大鼠脑垂体和脑中分布的研究。通过免疫荧光染色的方法绘制区域图，并通过免疫测定法在提取、凝胶过滤和离子交换层析之后确定反应肽。垂体两叶中的主要免疫反应性肽是C ′-片段和N-乙酰基C ′-片段，它们作为阿片类药物具有弱活性或无活性; C-片段及其N-乙酰基衍生物代表次要组分。这表明，在大鼠中，从垂体释放的循环“内啡肽”几乎没有吗啡样活性。在大鼠脑内也观察到相同的四种免疫反应肽。在海马中，C ′-片段是主要成分;在中脑中，C-片段较多，但C ′-片段占主导地位;在下丘脑中，C-片段是主要肽，几乎排除了其他肽。结果表明，在中枢神经系统的解剖学不同区域中，促脂素的加工受到差异控制。促脂素在下丘脑中的加工特别针对促脂素C片段的产生。
The recent isolation and identification of alpha-N-acetyl forms of the C-Fragment of lipotropin (beta-endorphin, residues 61-91) and the C'-Fragment (residues 61-87) [Smyth, D.G., Massey, D.E., Zakarian, S. & Finnie, M. (1979) Nature (London) 279, 252-254] has led to a study of their distribution in the pituitary and brain of the rat. Regions were mapped by the method of immunofluorescent staining and the reactive peptides were determined by immunoassay after extraction, gel filtration, and ion exchange chromatography. The major immunoreactive peptides in both lobes of the pituitary were found to be C'-Fragment and N-acetyl C'-Fragment, which are weakly active or inactive as opiates; the C-Fragment and its N-acetyl derivative represented minor components. This indicates that in the rat the circulating "endorphins" released from pituitary would have little morphinomimetic activity. The same four immunoreactive peptides were observed in rat brain. In the hippocampus the C'-Fragment was the principal component in the midbrain there was more C-Fragment but C'-Fragment predominated; in the hypothalamus the C-Fragment was the major peptide, almost to the exclusion of the other peptides. The results demonstrate that the processing of lipotropin is under differential control in anatomically distinct regions of the central nervous system. The processing of lipotropin in the hypothalamus is directed specifically to the production of lipotropin C-Fragment.