ISOLATION AND STRUCTURE OF 2 PROSTAGLANDIN ENDOPEROXIDES THAT CAUSE PLATELET-AGGREGATION
ISOLATION AND STRUCTURE OF 2 PROSTAGLANDIN ENDOPEROXIDES THAT CAUSE PLATELET-AGGREGATION
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DOI：
10.1073/pnas.71.2.345
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发表时间：
1974-01-01
影响因子：
11.1
通讯作者：
SAMUELSSON, B
中科院分区：
文献类型：
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作者：
HAMBERG, M;SVENSSON, J;SAMUELSSON, B
在1 mMP-苯甲酸汞存在下，将花生四烯酸与绵羊泡状腺匀浆的微粒体部分一起孵育短时间，然后进行提取和柱层析，得到两种前列腺素内过氧化物。这些化合物的结构，即，15-羟基-9 α，11 α-过氧化前列腺-5，13-二烯酸（前列腺素G2）和15-羟基-9 α，11 α-过氧化前列腺-5，13-二烯酸（前列腺素H2）主要通过化学转化为以前已知的前列腺素而归属。在灌流的主动脉条上，新的前列腺素比前列腺素E_2的活性高50-200倍（前列腺素G_2）和100-450倍（前列腺素H_2）。前列腺素内过氧化物在水介质中的半衰期（约5 min）明显长于豚鼠肺释放的“兔肺收缩物质”的半衰期，表明没有一种前列腺素内过氧化物与该因子相同。将10-300 ng/ml的内过氧化物添加到洗涤的人血小板的悬浮液中导致快速聚集。此外，凝血酶诱导的血小板聚集伴随着可被氯化亚锡还原为前列腺素F2α的物质的释放，从而表明内源性前列腺素内过氧化物参与了血小板聚集。
Incubation for a short time of arachidonic acid with the microsomal fraction of a homogenate of the vesicular gland of sheep in the presence of 1 mMp-mercuribenzoate followed by extraction and silicic acid chromatography yielded two prostaglandin endoperoxides. The structures of these compounds, i.e., 15-hydroperoxy-9α,11α-peroxidoprosta-5,13-dienoic acid (prostaglandin G2) and 15-hydroxy-9α,11α-peroxidoprosta-5,13-dienoic acid (prostaglandin H2), were assigned mainly by a number of chemical transformations into previously known prostaglandins. The new prostaglandins were 50-200 times (prostaglandin G2) and 100-450 times (prostaglandin H2) more active than prostaglandin E2on the superfused aorta strip. The half-life of the prostaglandin endoperoxides in aqueous medium (about 5 min) was significantly longer than that of “rabbit aorta-contracting substance” released from guinea pig lung, indicating that none of the prostaglandin endoperoxides is identical with this factor. Addition of 10-300 ng/ml of the endoperoxides to suspensions of washed human platelets resulted in rapid aggregation. Furthermore, platelet aggregation induced by thrombin was accompanied by release of material reducible by stannous chloride into prostaglandin F2α, thus indicating the involvement of endogenous prostaglandin endoperoxides in platelet aggregation.