Targeting of different ethiodized oil-doxorubicin mixtures to hypovascular hepatic metastases with intraarterial and intraportal injections
Targeting of different ethiodized oil-doxorubicin mixtures to hypovascular hepatic metastases with intraarterial and intraportal injections
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DOI：
10.1016/s1051-0443(96)72880-4
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发表时间：
1996-05-01
影响因子：
2.9
通讯作者：
Clouse, ME
中科院分区：
文献类型：
--
作者：
Cay, O;Kruskal, J;Clouse, ME
目得：在动脉内或门静脉内注射后，比较不同乙碘油-多柔比星混合物在无胸腺裸鼠中的少血管肝转移瘤内和周围的分布。阿霉素和乙碘油在水溶性造影剂中的六种不同混合物，分别振摇5、10、15和30分钟，制备并评估混合物中油滴的大小，在携带少血供肝结直肠转移瘤的小鼠中进行动脉内和门静脉内注射振摇10和15分钟的混合物。在体内视频显微镜被用来评估微血管流量和生物分布和大小的油滴内和周围的tumors.Results：直径小于20 μ m的油滴被肿瘤结节。动脉内和门静脉内注射之间肿瘤内油滴积聚没有显著差异。大于20 μ m的油滴阻塞中小型门脉分支。最佳组合为0.1 mL乙碘油溶于2 mL泛影葡胺钠（Renografin-76）中，以800 rpm振荡15 min，形成小于20 μ m的油滴（92%）。结论：含小于20 μ m油滴的乙碘油-抗癌药物混合物可栓塞肝转移瘤的无血管区。由于这些液滴通过动脉内或门静脉内注射渗透到肿瘤组织，因此通过动脉途径的化疗栓塞似乎更适合治疗不可切除的少血供肝转移，因为它更容易执行。本研究的结论仅限于实验性少血供肝转移瘤的动物模型。
PURPOSE: The distribution of different ethiodized oil-doxorubicin mixtures within and around hypovascular liver metastases in athymic nude mice was compared following either intraarterial or intraportal injections.MATERIALS AND METHODS: Six different mixtures of doxorubicin and ethiodized oil in water-soluble contrast material, shaken for 5, 10, 15, and 30 minutes, respectively, were prepared and the size of the oil droplets in the mixtures evaluated, Intraarterial and intraportal injections of mixtures shaken for 10 and 15 minutes were performed in mice bearing hypovascular hepatic colorectal metastases. In vivo video microscopy was used to evaluate microvascular flow and the biodistribution and the size of the oil droplets within and around the tumors.RESULTS: Oil droplets smaller than 20 mu m in diameter were taken up by tumor nodules. There was no significant difference in oil droplet accumulation within the tumor between intraarterial and intraportal injections. Oil droplets larger than 20 mu m occluded small and medium portal branches. The optimum combination was 0.1 mL of ethiodized oil in 2 mL of diatrizoate sodium meglumine (Renografin-76) shaken for 15 minutes at 800 rpm, which formed droplets smaller than 20 mu m (92%).CONCLUSION: Avascular regions of hepatic metastases may be embolized with ethiodized oil-anticancer drug mixtures containing oil droplets smaller than 20 mu m. Since these droplets penetrate to the tumor interstitium with either intraarterial or intraportal injections, chemoembolization via the arterial route seems preferable for treatment of unresectable hypovascular hepatic metastases since it is easier to perform. The conclusions drawn from this study are limited to the animal model for experimental hypovascular hepatic metastases.