Isolation of pathogenic fungi causing postharvest decay in table grapes and in vivo biocontrol activity of selected yeasts against them
Isolation of pathogenic fungi causing postharvest decay in table grapes and in vivo biocontrol activity of selected yeasts against them
复制标题
导致鲜食葡萄采后腐烂的病原真菌的分离以及所选酵母对其的体内生物防治活性
DOI：
10.1016/j.pmpp.2020.101478
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发表时间：
2020-04-01
影响因子：
2.7
通讯作者：
Zhang, Hongyin
中科院分区：
文献类型：
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作者：
Solairaj, Dhanasekaran;Legrand, Ngolong Ngea Guillaume;Zhang, Hongyin
由于致病真菌腐烂，鲜食葡萄采后损失越来越受到关注。为鉴定镇江地区鲜食葡萄腐烂病的病原真菌，对采自不同产地的鲜食葡萄进行了真菌分离。从葡萄果实采后病原菌中分离鉴定出日本曲霉（Aspergillus japonicas）（A3）、葡萄汁曲霉（Aspergillus uvarum）（B2）、枝孢霉（Cladosporium cladosporioides）（C3）、棘孢曲霉（Aspergillus aculeatus）（C4）、皱纹篮状菌（Talaromyces rugulosus）（C5）、乔治青霉（Penicillium georgiense）（C7）和扩展青霉（Penicillium expansum）7种真菌。在分离的真菌中，C.枝孢霉属的致病能力和致病潜力最大。据我们所知，这项研究代表了第一次从腐烂的葡萄中分离到的P.georgiense，并证实了其在鲜食葡萄中的致病潜力。此外，还研究了酿酒酵母、沼泽红孢子菌、河流红孢子菌和Wickerhamomyces paludigenum的生防能力。酵母菌在真菌菌株的基础上表现出不同程度的生防效果。R.对所有分离的真菌，河生菌表现出最高的生防效果。本研究将丰富对鲜食葡萄采后病原真菌的认识，并为葡萄采后腐烂病的生物防治提供新的数据。
There is an increasing concern about the postharvest loss of table grapes due to pathogenic fungal decay. In order to identify the potential pathogenic fungi causing decay in table grapes at Zhenjiang, Jiangsu Province, China, we aimed to isolate fungi from decayed table grapes. Seven fungi, namely Aspergillus japonicas (A3), Aspergillus uvarum (B2), Cladosporium cladosporioides (C3), Aspergillus aculeatus (C4), Talaromyces rugulosus (C5), Penicillium georgiense (C7) and Penicillium expansum were isolated and identified as postharvest pathogens of grape berries. Among the isolated fungi, C. cladosporioides has the highest potential of disease-causing ability and pathogenic ability. To the best of our knowledge, this study represents the first isolation of P. georgiense from decayed grapes and confirmed its pathogenic potential in table grapes. In addition, the biocontrol ability of Saccharomyces cerevisiae , Rhodosporidium paludigenum , Rhodosporidium fluviale and Wickerhamomyces anomalus was examined against the isolated fungi. The yeast strains showed various levels biocontrol efficacy based on fungal strains. R. fluviale showed the highest biocontrol efficacy against all the isolated fungi. The present study will enrich the knowledge in postharvest fungal pathogens of table grapes as well as provides novel data in the field of biological control of postharvest decay of grapes.