Differential gene expression pattern of diabetic rat retinas after intravitreal injection of erythropoietin
Differential gene expression pattern of diabetic rat retinas after intravitreal injection of erythropoietin
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DOI：
10.1111/j.1442-9071.2010.02437.x
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发表时间：
2011-03-01
影响因子：
4
通讯作者：
Xu, Guo-Tong
中科院分区：
文献类型：
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作者：
Chu, Qing;Zhang, Jingfa;Xu, Guo-Tong
目的：探讨糖尿病大鼠视网膜中促红细胞生成素（EPO）基因表达的变化。方法：采用链脲佐菌素（STZ）诱导的糖尿病大鼠，玻璃体内注射EPO后，收集视网膜神经感觉区，观察EPO对视网膜基因表达的影响。在糖尿病发病后0、30和120天进行促红细胞生成素玻璃体内注射。在上述时间点每次注射后4天，收获视网膜用于微阵列测定。结果：促红细胞生成素治疗后，糖尿病视网膜中上调的白细胞介素-2和白细胞介素-11等炎症因子的编码基因得到恢复。促凋亡效应基因Tnfrsf 5、Bid 3和Bcl 2l 1在糖尿病大鼠中表达上调，而在促红细胞生成素治疗组中表达减弱。此外，实时荧光定量PCR被用来确认基因Trex 2，G1 P2，DHX 58，RGD 1311906和LOC 689064的变化，这还没有被报道在糖尿病retinopathy.Conclusions：玻璃体内促红细胞生成素治疗是能够正常化的基因表达负责促凋亡和炎症反应注意到糖尿病视网膜。
P>Background:To profile the pattern of gene expression in diabetic rat retinas with or without intravitreal injection of erythropoietin.Design:By using streptozotocin-induced diabetic rats, after intravitreal injection of erythropoietin, neurosensory retinas were collected to determine the effect of erythropoietin on gene expression.Participants:Three groups of Sprague-Dawley rats were studied: normal control (15), diabetic rats with saline injection (15) and diabetic rats with intravitreal erythropoietin treatment (15).Methods:Diabetes was induced by intra-peritoneal injection of streptozotocin. Intravitreal injection of erythropoietin was performed at the following time points: 0, 30 and 120 days after diabetes onset. Four days after each injection at above-mentioned time points, the retinas were harvested for microarray assay. The real-time PCR was used to evaluate the microarray data.Results:Genes encoding inflammatory factors, such as interleukin-2 and interleukin-11, which were upregulated in the diabetic retinas, were restored after erythropoietin treatment. Genes encoding pro-apoptotic effectors, like Tnfrsf5, Bid3 and Bcl2l1, were also upregulated in diabetic rats and attenuated in erythropoietin-treated group. In addition, real-time PCR were employed to confirm the changes of the genes Trex2, G1P2, DHX58, RGD1311906 and LOC689064, which have not been reported in diabetic retinopathy.Conclusions:Intravitreal erythropoietin treatment is able to normalize the gene expression responsible for pro-apoptotic and inflammatory responses noted in diabetic retinas.